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E. Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠杆菌中分离多核苷酸■酸化酶的工作。

E.Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠桿菌中分离多核苷酸砱酸化酶的工作。

To study the effects E.coli purine nucleoside phosphorylase suicide gene on human gastric cancer MKN-45 cells as well as the bystander effect.

目的：探讨大肠杆菌嘌呤核苷磷酸化酶自杀基因对胃癌细胞MKN-45的抑制及杀伤作用，并证实旁观者效应。

In this paper, we studied the effect of sterilization on different bacteria and cyanobacteria in variant water sample of micro-electrolysis and high voltage electrostatic field, and the effect of physiological activity on E.coli and the characteristic optical absorption peak of phycocyanin of micro-electrolysis. The mechanism of sterilization and algae killing was approached.

本文研究了微电解、高压静电场作用对于不同水样中的不同种类的细菌和蓝藻的杀灭作用，以及微电解作用对于大肠杆菌的生理活性和对于蓝藻细胞内藻胆蛋白的光吸收特性的影响，探讨了杀菌灭藻的机理。

The expression of phycoerythrin beta and alfa subunit genes in E.coli and its activity study was done and will be done further.

另进行了龙须菜藻红蛋白b和a亚基基因在大肠杆菌体系中的蛋白质表达及蛋白活性研究。

A separated pathogenetic material of edema disease of pigling from endo-toxin is isolated, which is prepared from hemolytic E coli O139:K82 of the natural edema disease cases, by spreading culture, repeatedly freezing and thawing, rapidly centrifugalizing, concentrating and doing sephadexG-200 column chromatography.

本文主要报道从仔猪水肿病自然病例分离的溶血性大肠杆菌O_(130)：K_(82)，通过扩大培养，反复冻融，离心、浓缩，进行SephadexG-200柱层析，分离出了一种与内毒素分开的仔猪水肿病致病物质。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

Avian Escherichia coli ; type 1 pili ; structural gene ; cloning

鸡大肠杆菌； 1型菌毛；结构基因；克隆

The antibacterial effects of ethanol extract of Rhynchosia volubilis Lour roots on staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and pneumobacillus were observed.

方法（1）鹿藿醇提取物按照国际计划生育基金会认可的杀精子实验方法，对10只小鼠附睾精子悬液和10例人精液进行体外杀精实验。

The lowest inhibiting concentration of ethanol extract of Rhynchosia volubilis Lour roots on staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and pneumobacillus were 1.0,1.0,4.0,4.0 mg/ml,respectively.

结果鹿藿醇提取物对小鼠和人精子最低杀精浓度分别为50和100 mg/ml；对标准菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌MIC分别为1.0，1.0，4.0，4.0 mg/ml。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。