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The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

The prevalence of extended-spectrum betalactamase of Escherichia coli and Klebsilla spp. was 59.50% and 55.98%, respectively. 63.97% of Pseudo monas aeruginosa were isolated from sputum, drug-resistant rate of Pseudomonas aeruginosa in sputum from pa tients in ICU, neurosurgical department, respiratory and emergency pediatric department, respiratory department was 21.05%, 15.99%, 7.09% and 6.07% respectively.

大肠埃希菌和克雷伯菌属超广谱β-内酰胺酶产生率分别为59.50%和55.98%。63.97%的铜绿假单胞菌来源于痰标本，不同科室痰标本中分离的铜绿假单胞菌耐药率从高到低依次为重症监护室(21.05%)、神经外科(15.99%)、呼吸急救儿科(7.09%)、呼吸内科(6.07%)。

Moschus berezovskii IL-2 was cloned from Con-A stimulated peripheral blood mononuclear cells, and ligated with pIVID18-T vector, then transformed to Escherichia coli JM109. The positive recombinant was sequenced.

用林麝外周血单核细胞(peripheral blood mononuclear cells， PBMC)提取总RNA，RT-PCR扩增IL-2(interleukin-2， IL-2)，连接pMD18-T载体后转化大肠杆菌，筛选阳性克隆并测序。

Results The recombinant MPT 64 protein existed in inclusion bodies of E.coli.and amounted to 20%- 30% of totle cell protein.

具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。

Objective To explore the cell immunity of the 3rd stage larva of Musca domestica after being infected by Escherichia coli.

目的探讨家蝇3龄幼虫被大肠杆菌感染后的细胞免疫反应。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

The result of specificity showed that different serotypes of strains were all positive for stx2 gene,while nonpathogenic E.coli ATCC23716 was negative.

特异度的测定结果表明不同血清型的大肠埃希菌均为stx2阳性，而非致病性大肠埃希菌ATCC23716则为阴性。

Objective: To clone the complete gene of nucleoprotein of H3N2 subtype avian influenza virus, and to express recombinant NP in E.coli for the function research.

目的：获得H3N2亚型禽流感病毒核蛋白全长基因，并在大肠杆菌中表达，以用于对NP功能的研究。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。