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Class Ⅰ integrase genes are widespread in clinical isolates of Escherichia coli and Klebsiella.

Ⅰ类整合子在大肠埃希菌和克雷伯菌中分布广泛。

It was appeared that E.coli and Klebsiella produced extended-spectrum β-lactamases.

目的研究腹腔感染患者致病菌感染谱和耐药性的变化。

Objective To purify recombinant heat labile enterotoxin B subunit of Escherichia coli.

目的 纯化基因重组表达的大肠杆菌不耐热肠毒素B亚单位。

The main researches of this dissertation are summarized as follows:1. AFM study of different effects of natural and semisyntheticβ-lactam on the cell envelope of E.coli.

主要内容归纳如下：1、基于AFM大气氛中成像，研究天然与半合成内酰胺类抗生素对大肠杆菌形态的影响。

Determination of the properties of lactase expressed in E.coli.

原核表达乳糖酶的酶学性质测定制备原核表达乳糖酶的原酶液。

Cloning and expression in E.coli of lactase.

研究内容及结果如下： 1。

According to the analysis of thermogenic curves, shortened lag phase of bacteria growth, increased growth rate constant and maximum heat power were observed in the presence of Pr(superscript 3+) in low concentration and the results indicated that the growth of E. coli was promoted.

从生成的热谱图分析可以看出，低剂量的稀土离子Pr(上标 3+)的存在可以缩短细菌的停滞期，增加生长速率常数和最大放热功率，刺激大肠杆菌的生长。

Experimental results indicated that the silver nanoparticles of 20 μg/mL could inhibit completely the growth of 10^6 cfu/mL cells in liquid LB medium. The growth curves showed that silver nanoparticles prolonged the lag phase of E. coli, and the higher of the concentration of silver nanoparticles, the longer of the lag phase of E.

纳米银对大肠杆菌的抑制生长曲线的结果表明，20μg/mL的纳米银能够完全抑制10^6 cfu/mL的大肠杆菌细胞生长，纳米银使大肠杆菌的延滞期加长，并且纳米银浓度越高，延滞期越长。

The genes gB,gC and gD amplified from infectious laryngotracheitis virus Chinese Wanggang strain were cloned into the Eco RⅠ site of the eukaryotic expression vector pCAGGS,re-spectively. The positive recombinant plasmids were screened by transforming into E.coli DH5 α cells,which were named pCAGG-gB,pCAGG-gC and pCAGG-gD respectively after identification by restriction endonuclease analysis and sequencing analysis.

将传染性喉气管炎病毒王岗株的gB、gC、gD基因分别克隆到真核表达载体pCAGGS的 Eco RⅠ位点，经过酶切、测序分析，筛选鉴定出含有gB、gC、gD基因的重组质粒，分别命名为pCAGG-gB、pCAGG-gC、pCAGG-gD。

Both 50% boiling water extract and 50% alcohol extract of Zingiber Coral- linum Hance do not inhibtte the five tested bactreial species:Staphy.aureus, Strept.hemolytieus,B.subtilis,E.coli and S.typhus.

本文报告对珊瑚姜抑制细菌和真菌作用的研究。结果表明珊瑚姜50%水煎剂和50%酒精浸液对金黄色葡萄球菌、乙型溶血性链球菌、枯草杆菌、大肠杆菌和伤寒杆菌都无抑制作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。