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Its bacteriostasis in vitro showed that it could not inhibit the growth of E. coli in vitro.

体外抑菌实验结果表明:硫酸化葡聚糖在体外对大肠杆菌的生长没有抑制作用。

Preparation of a number of beef extract peptone ordinary medium, for different concentrations of nano-silver iodide solution were applied revealed pulp with a number of medium and E. coli in these uniform coating medium.

制备多个牛肉膏蛋白胨普通培养基,取不同浓度的纳米碘化银大白浆溶液分别涂抹与多个培养基上并将大肠杆菌均匀涂布于这些培养基中。

Coli. These studies generally used 400 500 mg berberine one to three times per day.

大肠杆菌。这些研究一般采用400-500毫克黄连素1至3倍,每天。

Methods Cadmium-specific promoter PcadA was obtained by PCR from pPcadA and was ligated to E.coli-B.subtilis shuttle vector pNGFP with gfp being reporter gene ,after which the recombinant plasmid was transformed into 1A751 strain to form the bio-engineered bacteria.

采用聚合酶链式反应从质粒pPcadA上扩增得到重金属镉离子特异性启动子PcadA,以绿色荧光蛋白基因为报告基因,以大肠杆菌-枯草杆菌穿梭载体pNGFP构建了绿色荧光蛋白镉离子诱导表达载体。

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本实验从抗除草剂转基因Bobwhite小麦中,利用PCR克隆的方法扩增出bar基因全长,并在原核表达系统中表达,鉴定表达蛋白的活性,将能够正确编码PPT乙酰转移酶的bar基因片段,经过适当的修饰构建入真核表达载体。

A mutant Escherichia coli strain in which the bolA gene was knocked out was generated by homozygous recombination method.

表明水稻OsBolA1蛋白主要在细胞核内发挥功能,可能作为转录调控因子起作用。

Rice OsbolA1 gene encodes a predicted protein with 167 amino acids showing 52% sequence identity with Escherichia coli BolA protein.

水稻OsbolA1 cDNA含501bp的ORF,编码167aa,推导的氨基酸序列与大肠杆菌BolA有52%的同源性。

It provides reference to prevention and treatment of broiler pathogenic E coli in this area.

试验结果对指导潍坊地区肉鸡大肠杆菌病防治具有参考价值。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型,检测气道压力峰值—时间指数、支气管/肺泡灌洗液中炎性细胞数量,PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平,免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Coli were purified and used as the coating antigen in indirect ELISA test, and the results were compared with those obtained by the traditional tube agglutination test method in 100 serum samples in general survey for the Brucella infection.

以大肠杆菌表达、纯化的BP26和OMP31重组蛋白为包被抗原,初步建立了检测血清特异抗体的间接ELISA方法,并以现地牛布氏杆菌普查检测血清100份为样本,与传统的试管凝集试验进行了比较研究。

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