查询词典 coli

Recombinant colony, E.coli DH5α(pMG320),expressing O\|antigen of V.cholerae O 139 was detected from the bank by immunological agglutinative reaction.

经鉴定分析重组克隆所表达的O 抗原具有良好的免疫原性及反应原性。酶切分析质粒 pMG32 0 ，推知其O 抗原基因大小约 4 6kb。

Chickens were experimentally infected with Mycoplasma gallisepticum and Escherichia coli via the air sac.

雏鸡气囊人工混合感染鸡毒支原体和大肠杆菌。

Using recombinant E. coli to produce high-yield ALA is a new and promising technology.

利用基因重组技术实现ALA的批量生产是合成ALA的新技术。

MethodWith Staphylococcus aureus,Escherichia coli and Bacillus subtilis as tested bacterium,the bacteriostatic effects of ethanol extracts from Alsophila spinulosa leaves and stems on 3 kinds of bacteria were studied and compared with that of coptis extracts.

方法以金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌为供试菌种，研究了桫椤茎叶和茎干甲醇提取物对它们的抑菌作用，并与黄连提取物的抑菌效果进行比较。

Futher results showed that: the optimum growth temperature for the 4 lactic acid bacteria was 30℃; and the pH 3.5~4.0; all 4 lactic acid bacteria could grow at the medium with 10% NaCl, and the optimum range was 2%~6% They were aerotolerant anaerobe; They showed certain inhibition on E coli..

进一步测试表明：4株乳酸菌在30℃培养时，生长最好；在pH3.5~4.0时生长良好；4株乳酸菌在10% NaCl的环境中均能生长，其中以2%~6%的NaCl浓度内生长最适宜；属兼性厌氧菌；对大肠杆菌产生一定的抑制作用。

Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌，采用Tn5转座子诱变技术，以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌，使Tn5插入并整合到冰核细菌基因组中，使冰核基因的线性连续性被破坏，失去冰核活性。

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon，邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高，为高产色氨酸基因工程菌的构建奠定了基础。

The complex can improve the antibiosis activity to Escherichia coli.

配合物能提高配体对大肠杆菌的抗菌活性。

Expression of nitroreductase gene nor_1 in e.coli and the preparation of antiserum.

NOR1基因真核表达载体的构建及其对肝癌细胞生长的影响。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失