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Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

In principle, the protocol developed in this study could be used for detecting other foodborne pathogenic bacteria such as E.coli, Staphylococcus, Campylobacter and food toxins such as Ricin, Abrin, or Clostridium Botulinum if the proper antibody is selected for the conjugation with nanorod substrates.

大致上，这项研究兴盛的草案可以通过选择性结合在纳米棒底层的相对性抗体被用于检测另外一些食源性病原菌，比如大肠盖希氏菌，葡萄球菌，弯曲杆菌和食物毒素，诸如蓖麻毒素，红豆因或肉毒梭菌。

1 The scavenging effects of fucoidans on superoxide, hydroxyl radical and hypochlorous acid were studied by the method of chemiluminescence and Escherichia.coli.

发现低分子量的岩藻聚糖硫酸醋对LDL有很好的抑制作用，活性明显高于大分子的组份，而且硫酸根含t越高，抗氧化效果越好。

The minimal bactericidal concentration of 40% EtOH extractum for Escherichia coli was 6.25mg/mL, for Staphylococcus aureus, Salmonella and Pasteurella multocida was 12.25 mg/mL, for Streptococcus agalactia was 25.00mg/mL.

对金黄色葡萄球菌、沙门氏菌和多杀性巴氏杆菌的MBC均为12.25mg/mL，对无乳链球菌的MBC为25.00mg/mL。

To date, only the structures of prokaryotic succinate:ubiquinone oxidoreductases, which share a similar enzymatic function with mitochondrial SQR, have been reported, including QFR from E.coli, QFR from Wolinella succinogenes and SQR from E.coli.

在此之前，只有原核生物的琥珀酸泛醌氧化还原酶的结构得到了解析，这包括大肠杆菌的 QFR ， Wolinella succinogenes 细菌中的 QFR 和大肠杆菌的 SQR ，这些蛋白有着与线粒体复合物 II 相类似的酶功能。

Three standard and two isolated strains of E.coli were used for drug sensitivity test on Chloramphenicol, Ciprofloxacin Lactate, Rifampicin, sulfamonomethoxine, Florfenicol, Ceftiofur Sodium as well as the antimicrobial synergists sulbactam sodium and TMP. The bacteriostasis results of the antibiotics against E.coli In vitro were compared when they were used separately or combined with the synergist.

试验用3株大肠杆菌标准株和2株地方分离株，选择氯霉素、乳酸环丙沙星、利福平、磺胺-6-甲氧嘧啶钠、氟苯尼考、头孢噻呋和抗菌增效剂舒巴坦钠、TMP进行药敏试验，比较几种药物单一或与增效剂合用对大肠杆菌的体外抑菌效果。

The real-time PCR results show that 10 isolates were amplified while 54 isolates did not produce considerable amplification. So that , the 10 isolates were identified as E coli O157 and the 54 isolates were non- E coli O157, which comply with the results of serotyping.

说明出现扩增曲线的10株细菌是大肠杆菌0157，未出现指数扩增的54株细菌是非0157大肠杆菌，荧光定量PCR法和血清学检测结果完全一致。

In addition, we compared the codon preferences among poplar, Arabidopsis thaliana, Oryza sative, Homo sapi- ens and Escherichia coli, and poplar was found to be most similar to A.thaliana and least similar to E.coli.

将杨树的密码子使用频率与拟南芥、水稻、大肠杆菌和人等不同模式生物种比较后发现，杨树密码子的偏爱性与同为双子叶植物的拟南芥最为相似，与人和大肠杆菌之间的差异较大。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失