查询词典 coli

In the end, magnetic water was used to cultivate Escherichia-coli, the different effect on Escherichia-coli of magnetic wate and unmagnetized tri-distilled was compared by electrophoretic analysis.

这些无定形结构的分子簇可以经一定的物理、化学处理，使其转化为较小的分子簇。磁化，作为简单而用实际的方法，被大多数实验室采用，用以进行对&水簇&的研究。

Furthermore. the bilirubin oxsidase activity was detected in the supernatant of cell lysates from IPTG induced culture of E. coli containing pEI-3a/BOX These results demonstrated that bilirubin oxidase cDNA from Myrothecium Verrucaria has been successfully expressed in E. coli.

这些结果表明，疣疱漆斑菌胆红素氧化酶cDNA首次成功地在大肠杆菌中获得了表达，所表达的可溶性产物具有与天然蛋白质基本相同的分子量、相似的免疫性和酶催化活性

According to the amino acid sequence of monellin and the bias in coden choice in Escheria coli,a single chain monellin gene was synthesized and subcloned into a Escheria coli expression vector pET-28a.

根据已报道的单链Monellin甜蛋白的氨基酸序列，按大肠杆菌基因偏爱密码子，设计和人工合成了单链monellin基因。

Actually, some pessimists give E. coli 100-to-1 odds on outliving humans, even though E. coli can currently live only in our guts.

事实上，在大肠杆菌和人类谁更长寿的赌局上，许多悲观主义者给出大肠杆菌100比1的赔率，尽管现在，这种微不足道的生物只能生存在我们人类的身体里。

And neither particularly has an advantage over the other in surviving the next million years. Actually, some pessimists give E. coli 100-to-1 odds on outliving humans, even though E. coli can currently live only in our guts.

不管是现代人类还是大肠杆菌，都是进化的幸存者，是经历了亿万年淘汰后胜利的佼佼者。

Since the post-translational modification process doesn't exist in E. Coli expression system, in order to investigate if the natural nsLTP has antimicrobial activity, we purified nsLTP from rice etiolated seedlings. They were shown to have the similar antifungal activity compared with E. Coli expressed nsLTP.

由于大肠杆菌表达产物缺少翻译后加工，为了与表达产物的功能研究相对照，还摸索了从水稻黄化苗中抽提nsLTP的方法，结果表明水稻苗中提取的nsLTP亦具有抑菌活性，抗性水平接近大肠杆菌表达产物。

Construction of E. coli transformants overproducing S1 and GDHTo express the carbonyl reductase S1 and GDH genes in the same E. coli cells, four expression vectors were constructed Fig.

为ㄋ在同─大肠杆菌细胞中表达碳酰还原酶S1和GDH基因，要构建四个表达型载体。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Acterial artificial chromosome has several notable advantages, such as inserting large fragments, maintaining the stability of inserted DNA in E.coli, producing few chrisms, recovering inserted DNA from E.coli cell and easy in library construction.

AC文库的构建是基因组较大的真核生物基因组学研究的重要基础，可用于真核生物重要基因及全基因组物理作图、重要性状基因的图位克隆、基因结构及功能分析。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失