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In principle, the protocol developed in this study could be used for detecting other foodborne pathogenic bacteria such as E.coli, Staphylococcus, Campylobacter and food toxins such as Ricin, Abrin, or Clostridium Botulinum if the proper antibody is selected for the conjugation with nanorod substrates.

大致上，这项研究兴盛的草案可以通过选择性结合在纳米棒底层的相对性抗体被用于检测另外一些食源性病原菌，比如大肠盖希氏菌，葡萄球菌，弯曲杆菌和食物毒素，诸如蓖麻毒素，红豆因或肉毒梭菌。

1 The scavenging effects of fucoidans on superoxide, hydroxyl radical and hypochlorous acid were studied by the method of chemiluminescence and Escherichia.coli.

发现低分子量的岩藻聚糖硫酸醋对LDL有很好的抑制作用，活性明显高于大分子的组份，而且硫酸根含t越高，抗氧化效果越好。

The minimal bactericidal concentration of 40% EtOH extractum for Escherichia coli was 6.25mg/mL, for Staphylococcus aureus, Salmonella and Pasteurella multocida was 12.25 mg/mL, for Streptococcus agalactia was 25.00mg/mL.

对金黄色葡萄球菌、沙门氏菌和多杀性巴氏杆菌的MBC均为12.25mg/mL，对无乳链球菌的MBC为25.00mg/mL。

Recombinant colony, E.coli DH5α(pMG320),expressing O\|antigen of V.cholerae O 139 was detected from the bank by immunological agglutinative reaction.

经鉴定分析重组克隆所表达的O 抗原具有良好的免疫原性及反应原性。酶切分析质粒 pMG32 0 ，推知其O 抗原基因大小约 4 6kb。

Chickens were experimentally infected with Mycoplasma gallisepticum and Escherichia coli via the air sac.

雏鸡气囊人工混合感染鸡毒支原体和大肠杆菌。

Using recombinant E. coli to produce high-yield ALA is a new and promising technology.

利用基因重组技术实现ALA的批量生产是合成ALA的新技术。

MethodWith Staphylococcus aureus,Escherichia coli and Bacillus subtilis as tested bacterium,the bacteriostatic effects of ethanol extracts from Alsophila spinulosa leaves and stems on 3 kinds of bacteria were studied and compared with that of coptis extracts.

方法以金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌为供试菌种，研究了桫椤茎叶和茎干甲醇提取物对它们的抑菌作用，并与黄连提取物的抑菌效果进行比较。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Futher results showed that: the optimum growth temperature for the 4 lactic acid bacteria was 30℃; and the pH 3.5~4.0; all 4 lactic acid bacteria could grow at the medium with 10% NaCl, and the optimum range was 2%~6% They were aerotolerant anaerobe; They showed certain inhibition on E coli..

进一步测试表明：4株乳酸菌在30℃培养时，生长最好；在pH3.5~4.0时生长良好；4株乳酸菌在10% NaCl的环境中均能生长，其中以2%~6%的NaCl浓度内生长最适宜；属兼性厌氧菌；对大肠杆菌产生一定的抑制作用。

Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌，采用Tn5转座子诱变技术，以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌，使Tn5插入并整合到冰核细菌基因组中，使冰核基因的线性连续性被破坏，失去冰核活性。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48