查询词典 coli-

Acterial artificial chromosome has several notable advantages, such as inserting large fragments, maintaining the stability of inserted DNA in E.coli, producing few chrisms, recovering inserted DNA from E.coli cell and easy in library construction.

AC文库的构建是基因组较大的真核生物基因组学研究的重要基础，可用于真核生物重要基因及全基因组物理作图、重要性状基因的图位克隆、基因结构及功能分析。

Finally, wetested its antibacterial activities by E.coli and S.aureus.Methods In this research, the recombinded gene was cloned into the secretion vector pTYB12 and expressed in the E.coli BL21DE3. We did the preliminary research on secretion-expression of the gene. During the purification procedure, we used the chintin beads to wash off the sundry protein, then dialysed the collecting liquid to get rid of the salinity and the other small molecular peptides.

将上述重组基因抗菌肽的氨基酸片段克隆并拼接到分泌表达载体pTYB12上转染大肠杆菌(E.coli BL21DE3)后进行分泌表达，破碎离心收集的诱导表达大肠杆菌，首次应用几丁质珠柱分离纯化系统进行分离纯化，分离洗脱杂蛋白后透析去除盐类物质及其它小分子肽，得到目的重组基因抗菌肽。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

To date, only the structures of prokaryotic succinate:ubiquinone oxidoreductases, which share a similar enzymatic function with mitochondrial SQR, have been reported, including QFR from E.coli, QFR from Wolinella succinogenes and SQR from E.coli.

在此之前，只有原核生物的琥珀酸泛醌氧化还原酶的结构得到了解析，这包括大肠杆菌的 QFR ， Wolinella succinogenes 细菌中的 QFR 和大肠杆菌的 SQR ，这些蛋白有着与线粒体复合物 II 相类似的酶功能。

Three standard and two isolated strains of E.coli were used for drug sensitivity test on Chloramphenicol, Ciprofloxacin Lactate, Rifampicin, sulfamonomethoxine, Florfenicol, Ceftiofur Sodium as well as the antimicrobial synergists sulbactam sodium and TMP. The bacteriostasis results of the antibiotics against E.coli In vitro were compared when they were used separately or combined with the synergist.

试验用3株大肠杆菌标准株和2株地方分离株，选择氯霉素、乳酸环丙沙星、利福平、磺胺-6-甲氧嘧啶钠、氟苯尼考、头孢噻呋和抗菌增效剂舒巴坦钠、TMP进行药敏试验，比较几种药物单一或与增效剂合用对大肠杆菌的体外抑菌效果。

The real-time PCR results show that 10 isolates were amplified while 54 isolates did not produce considerable amplification. So that , the 10 isolates were identified as E coli O157 and the 54 isolates were non- E coli O157, which comply with the results of serotyping.

说明出现扩增曲线的10株细菌是大肠杆菌0157，未出现指数扩增的54株细菌是非0157大肠杆菌，荧光定量PCR法和血清学检测结果完全一致。

In addition, we compared the codon preferences among poplar, Arabidopsis thaliana, Oryza sative, Homo sapi- ens and Escherichia coli, and poplar was found to be most similar to A.thaliana and least similar to E.coli.

将杨树的密码子使用频率与拟南芥、水稻、大肠杆菌和人等不同模式生物种比较后发现，杨树密码子的偏爱性与同为双子叶植物的拟南芥最为相似，与人和大肠杆菌之间的差异较大。

Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48