查询词典 coli-

Piglet edema were first discovered in Britain, is hemolytic E. coli intestinal toxemia, so capillaries or systemic damage to the small blood vessels, increasing permeability and fluid extravasation too much water, to the head, eyelids, Equality ear edema, acute ataxia and death as the main feature of acute infectious diseases, also known as gastrointestinal edema pigs or pig intestinal toxemia E. coli, a highly lethal disease.

仔猪水肿病最早发现于英国，是由溶血性大肠杆菌引起肠毒血症，而使全身毛细血管或小血管受到破坏，通透性增大，水液外渗过多，造成的以头部、眼睑、耳部等处水肿、共济失调和急性死亡为主要特征的急性传染病，又称猪胃肠水肿或猪大肠杆菌肠毒血症，有高度致死性疾病。

By induction of IPTG, the E.coli with pET28a-hnRNPA2/B1 expressed the fusion protein ,which stranded at the 41KD by SDS-PAGE analysisthe comparing to E.coli uninduced and the control .

并且融合蛋白以可溶蛋白和包涵体两种形式存在。

In the end, magnetic water was used to cultivate Escherichia-coli, the different effect on Escherichia-coli of magnetic wate and unmagnetized tri-distilled was compared by electrophoretic analysis.

这些无定形结构的分子簇可以经一定的物理、化学处理，使其转化为较小的分子簇。磁化，作为简单而用实际的方法，被大多数实验室采用，用以进行对&水簇&的研究。

Furthermore. the bilirubin oxsidase activity was detected in the supernatant of cell lysates from IPTG induced culture of E. coli containing pEI-3a/BOX These results demonstrated that bilirubin oxidase cDNA from Myrothecium Verrucaria has been successfully expressed in E. coli.

这些结果表明，疣疱漆斑菌胆红素氧化酶cDNA首次成功地在大肠杆菌中获得了表达，所表达的可溶性产物具有与天然蛋白质基本相同的分子量、相似的免疫性和酶催化活性

According to the amino acid sequence of monellin and the bias in coden choice in Escheria coli,a single chain monellin gene was synthesized and subcloned into a Escheria coli expression vector pET-28a.

根据已报道的单链Monellin甜蛋白的氨基酸序列，按大肠杆菌基因偏爱密码子，设计和人工合成了单链monellin基因。

Actually, some pessimists give E. coli 100-to-1 odds on outliving humans, even though E. coli can currently live only in our guts.

事实上，在大肠杆菌和人类谁更长寿的赌局上，许多悲观主义者给出大肠杆菌100比1的赔率，尽管现在，这种微不足道的生物只能生存在我们人类的身体里。

And neither particularly has an advantage over the other in surviving the next million years. Actually, some pessimists give E. coli 100-to-1 odds on outliving humans, even though E. coli can currently live only in our guts.

不管是现代人类还是大肠杆菌，都是进化的幸存者，是经历了亿万年淘汰后胜利的佼佼者。

Since the post-translational modification process doesn't exist in E. Coli expression system, in order to investigate if the natural nsLTP has antimicrobial activity, we purified nsLTP from rice etiolated seedlings. They were shown to have the similar antifungal activity compared with E. Coli expressed nsLTP.

由于大肠杆菌表达产物缺少翻译后加工，为了与表达产物的功能研究相对照，还摸索了从水稻黄化苗中抽提nsLTP的方法，结果表明水稻苗中提取的nsLTP亦具有抑菌活性，抗性水平接近大肠杆菌表达产物。

Construction of E. coli transformants overproducing S1 and GDHTo express the carbonyl reductase S1 and GDH genes in the same E. coli cells, four expression vectors were constructed Fig.

为ㄋ在同─大肠杆菌细胞中表达碳酰还原酶S1和GDH基因，要构建四个表达型载体。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48