查询词典 coli-

Object In this study we framed the refusion gene antibacterial peptide[ Cecropin A(l ~ 8)- Magainin (1 - 12),CA(1 ~ 8)-MA(l ~12),CAM] vetor and expressed it in the Escherichia.coli. Thenpurified the recombinded peptide in the Intein Mediated Purificationwith an Affinity Chitin-binding Tag-IMPACT system.

目的 构建重组基因抗菌肽[Cecropin A(1～8)-Magainin(1～12)，CA(1～8)-MA(1～12)，CAM]质粒载体并在大肠杆菌(Escherichia.coli)中表达，应用几丁质自切割分离纯化系统(InteinMediated Purification with an Affinity Chitin-binding Tag，IMPACT)分离纯化抗菌肽，并测定其抗菌活性。

Inclusion bodies are easily formed when recombinant proteins are expressed in E.coli system, and how to renature these inclusion bodies is now becoming the key problem in the genetic engineering.

E.coli作为目前应用最为广泛的原核表达系统，在异体表达蛋白质的过程中容易形成无活性的包涵体。

The antibacterial effects of ethanol extract of rhynchosia volubilis lour roots on staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and pneumobacillus were observed.

用试管法及琼脂平皿稀释法观察鹿藿醇提取物对几种临床常见致病菌的体外抑菌作用。

The lowest inhibiting concentration of ethanol extract of Rhynchosia volubilis Lour roots on staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and pneumobacillus were 1.0,1.0,4.0,4.0 mg/ml,respectively.

对临床分离菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌MIC分别为0.5，2.0，8.0 mg/ml。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

In this research, we isolated SbNHX1 gene from Salicornia Bigelovii, and expressed it in E. coli. Salt tolerant analysis has been done in the SbNHX1 expressing E.

本研究对从北美海蓬子中分离的Na+/H+逆向运输蛋白基因在微生物中进行了表达，并进行了耐盐分析。

The following results have been obtained: 1 Expression of SbNHX1 in E. coli enhanced Na+ and K+ tolerances, but not to others; 2 The sequence from 1353-1514bp in SbNHX1 gene played a role for SbNHX1 to Na+ and K+ tolerances; 3 The Na+/H+ antiporter of Salicornia Bigelovii Torr. catalysed Na+/H+ or K+/H+ exchange with equal affinity, which indicates that the SbNHX1 is more likely to locate on member of vacuolar, and responds to transport Na+ into the vacuole from cytoplasm.

证明了SbNHX1能抵抗Na+和K+的胁迫，但对其它盐离子没有抗性；2）证明了北美海蓬子Na+/H+逆向转运蛋白基因（SbNHX1）C末端从基因的C末端1353bp至1514bp的序列在耐盐中起一定的作用；3）证明了北美海蓬子Na+/H+逆向转运蛋白（SbNHX1）对Na+和K+交换的等亲和性，对于这一点，说明北美海蓬子Na+/H+逆向转运蛋白与定位于液泡膜上的Na+/H+逆向转运蛋白的功能相似。

Methods〕E coli and salmonellae were cultured together with different culturing media.Then the enriching effect of each enrichment medium was determined.

方法〕用一定数量的大肠埃希菌与不同数量种类的沙门菌混合培养，观察不同增菌液的增菌效果。

The antimicrobial activities were determined with paper disc diffusion method. The result showed that of Sapindus mukorossi extracts only inhibited Candida albicans and no antimicrobial activities to Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pityrosporum ovale and Propionibacterium acnes.

配制无患子溶液进行抗菌试验，以圆盘滤纸扩散法分析，结果显示甲、乙、丙、丁醇溶液样品，唯对白色念珠菌具抑制效果，而对另外5种菌种(大肠杆菌、金黄色葡萄球菌、绿脓杆菌、皮屑芽孢菌、痤疮杆菌)无明显抗菌效果。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。