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Coli LLO/OVA as its creating pore activity helping OVA antigen escape from lysosomal vesicle of dendritic cells, and the bacterial content can then be processed by the proteosome and the antigen motifs presented onto MHC class I to CD8+ T cells, initiating stronger cytotoxic T cell immunity.

本实验选用LLO作为免疫佐剂，利用其穿孔素作用促OVA抗原从树突状细胞的吞噬溶酶体进入细胞浆，并经MHC-I类分子递呈给CD8+ T细胞，从而诱导较强抗肿瘤免疫。

Chlamydia psittaci ; Escherichia coli heat-liable enterotoxin B subunit ; Major outer membrane protein ; Prokaryotic expression

鹦鹉热衣原体；大肠杆菌热不稳定肠毒素B亚单位；主要外膜蛋白；原核表达

Subtilis,Salmonella pullorum and Escherichia coli excluding inhibitive effects of H2O2 an...

所产抑菌物质具有较好的热稳定性，在酸性条件下稳定且活性高。

The inhibitory tests in vitro were performed against Escherichia coli, Salmonella pullorum with filter paper method and tube dilution method. And the diameters of inhibition zone, minimal inhibitory concentrations of levofloxacin combined with polymyxin E were measured.

方法］应用纸片法和试管稀释法进行体外抑菌试验，测定左氧氟沙星与多黏菌素联合对鸡大肠杆菌、鸡白痢沙门氏菌的抑菌圈大小和最小抑菌浓度。

The S. pullorum on the surface of shell eggs was completely killed and E. coli O157:H7 logarithm of counts decreased to less than 1.0 log10 by NEW containing 12 mg/L available chlorine for 3 min without viable cells in the washing water after treatment.

当中性电解水清洗消毒被人工污染的鸡蛋表面时，有效氯质量浓度为12 mg/L、处理3 min可将鸡蛋表面的鸡白痢沙门氏菌全部杀灭，大肠杆菌O157：H7菌落数对数降低到1.0 log10 以下，且处理废液中没有残存菌，无二次污染问题。

For the sake of developing an effective, safe and no pollution disinfectant to reduce or eliminate food-borne pathogens on shell eggs, the bactericidal activity of neutral electrolyzed water (NEW, pH 6.0-7.5) generated by electrolysis of diluted hydrochloric acid in a non-membrane electrolytic cell for inactivation of Salmonella pullorum, Escherichia coli O157:H7 and artificial inoculated shell eggs initial logarithm of population of two pathogens on the eggs were approximately 6.19-6.26 log10 (cfu/g and 6.12-6.19 log10 respectively was evaluated.

为寻求一种高效、安全、无污染的禽蛋清洗消毒剂，采用无隔膜电解装置电解稀盐酸溶液制备中性电解水（pH值6.0～7.5）考查不同有效氯浓度、处理时间和温度条件下中性电解水对鸡蛋人工接种鸡白痢沙门氏菌（Salmonella pullorum，鸡蛋表面的初始菌落数对数为6.19～6.26 log10）和大肠杆菌O157：H7（鸡蛋表面的初始菌落数对数为6.12～6.19 log10）的杀灭效果。

Coli lpdA mutant has the potential to produce pyruvic acid from xylose and mannose.

这些结果对构建以大肠杆菌为母体的模式&细胞工厂&有参考价值。

Results: The results showed that both the wild type of recombinant MLCK and the ATP binding site mutagenesis of MLCK expressed in E.coli quantifiably with a large amount and exist as a soluble form.

结果：实验结果显示重组MLCK和MLCK/△ATP在大肠杆菌中以可溶的形式大量表达；经CaM-Sepharose 4B和Superose 6 HR纯化，SDS-PAGE鉴定得到单一的表达条带。

For the analysis of the non-kinase activity and the relationship between kinase activity and non-kinase activity, the recombinant full length MLCK was expressed and purified quantifiably with the E.coli expression system. The ATP binding site site-directed mutants of MLCK which lost the kinase activity were acquired.

为了更好地研究MLCK分子的非激酶作用以及激酶与非激酶两者的关系，本实验利用大肠杆菌表达系统大量表达并纯化出完整的MLCK分子，并对其ATP结合位点进行定点突变，获得无激酶活性的MLCK突变体。

Antibiotic susceptibility tests were carried out by K-B diffusion method on 41 vibrio strains isolated for one year both from farming seawater (18 strains) and digestion guts (23 strains) of the cobia, Rachycentron canadum . We chose 10 different species of antibiotics and refered to NCCLS performance standards for antimicrobial susceptibility testing, while taking Staphylococcus aureus ATCC25923 and Esсheriсhia coli ATCC25922 to control the test.

选用常用的10种抗生素，通过纸片扩散法（Kirby-Bauer纸片扩散法，简称K-B法），参照NCCLS抗生素敏感试验操作标准，并以金黄色葡萄球菌 Staphylococcus aureus ATCC25923和大肠埃希菌Esсheriсhia coli ATCC25922为质控菌株，对周年监测分离自养殖军曹鱼水体及肠道的41株优势弧菌（水体菌18株，肠道菌23株）进行了药物敏感性研究。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。