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The results showed that the three scFvs specific for Xac were successfully expressed in E. coli HB2151. The molecular weight of expressed products was about 32.0×103 and the products were mainly located in the periplasm.

结果显示，3株抗柑桔溃疡病菌可溶性单链抗体在大肠杆菌HB2151中均获得成功表达，表达产物分子量约为32.0×10^3，主要集中于细菌周质腔中。

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

After infecting E.coli HB2151 cells with one positive clone,soluble recombinant antibodies about 27 kDa were produced and located in the periplasm and the supernatant.

约27kDa大小的ER以可溶形式存在于细胞质及细胞周质中，并可分泌至上清。

Coil and B. subtilis using the β-lactamase distribution assay. The results showed that the β-lactamase activities of E.coli are mainly in periplasm while the enzyme produced by B.

内酰胺酶活力测定表明，大肠杆菌产生的酶主要积累在周质空间，而枯草芽孢杆菌产生的酶主要分泌在胞外。

Western blotting confirmed that the low activity of b-lactamase in E. coli Top10 pcs+ resulted from a lower amount of b-lactamase in its periplasm.

由此可见，周质内低含量的b-内酰胺酶是导致E.coli Top10 pcs+细菌氨苄青霉素抗性降低的原因。

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达，对诱导剂IPTG的浓度和诱导温度进行了优化，通过降低诱导温度使产物由包含体变成可溶性融合型产物；利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#，对诱导时间进行了优化，获得了较高的表达量，但发现产物没有明显的分泌表达，绝大部分是以可溶形式存在于胞内。

The ethanol extracts of seaweed belonging to Chlorophyta,Rhodophyta and Phaeophyta ,collected from Shandong coast in May 2000,were assayed their activities against two kinds of bacteria of Escherichia coli and Staphylococcus aurens.

分属绿藻门、红藻门、褐藻门的海藻样品于 2 0 0 0年 5月采于山东青岛和威海附近沿海，将海藻样品的乙醇提取物进行薄层层析分离和抗大肠杆菌、金黄色葡萄球菌活性测定。

Finally, the fluorescent intensity was measured by flow cytometry. Results: The E. coli was labeled well by FITC in carbonate buffer. Macrophage can phagocytize E.

结果：FITC能够有效标记大肠埃希菌；小鼠腹腔诱导的巨噬细胞能够吞噬大肠埃希菌，并且在30min时达到最大峰值。

Finally, the fluorescent intensity was measured by flow cytometry. Results: E. coli was labeled well by FITC in carbonate buffer. Macrophage can phagocytize E.

结果：FITC能够有效标记大肠埃希菌；小鼠腹腔诱导的巨噬细胞能够吞噬大肠埃希菌，并且在30min时达到最大峰值。

Escherichia coli and many other microoganism synthesize aromatic amino acids through the condensation reaction between phospho enolpyruvate and erythro.

ppsA和tktA是芳香族氨基酸生物合成中心途径的两个关键酶基因，在大肠杆菌中，ppsA基因编码磷酸烯醇式丙酮酸合成酶A ，该酶催化丙酮酸合成磷酸烯醇式丙酮酸；tktA基因编码转酮酶A ，该酶在磷酸戊糖途径中生成 4 磷酸赤藓糖起主要作用。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。