查询词典 coli-

The detection rates of methicillin-resistant S. aureus and penicillin-nonsusceptible S. pneumoniae were 15.9% and 84.3%, respectively. The prevalence of ESBLs-producing strains in Klebsiella pneumoniae, Escherichia coli, Serratia spp and Enterobacter cloacae were 31.2%, 46.2%, 94.8% and 16.8% respectively. The resistance rates of Hemophilus influenzae and Hemophilus parainfluenzae to ampicilin were 40% and 36% respectively.

耐甲氧西林金黄色葡萄球菌检出率为15.9%；对青霉素不敏感的肺炎链球菌（包括PISP和PRSP）检出率为84.3%；肺炎克雷伯菌、大肠埃希菌、粘质沙雷菌和阴沟肠杆菌产ESBLs的检出率分别为31.2%、46.2%、94.8%和16.8%；流感嗜血杆菌和副流感嗜血杆菌对氨苄西林的耐药率为36%和40%；铜绿假单胞菌和鲍曼复合不动杆菌对亚胺培南的耐药率分别为10.7%和13.2%。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

There is a new option to construct E.coli strain for efficient expression of heterologous proteins.

为改造大肠杆菌，提高外源蛋白的功能性表达作了初步的探索。

The result indicated that the cellular fatty acid compositions of Salmonella, Escherichia coli, Vibrio and Shigella all contain tetradecanoic acid, hexadecanoic acid and octadecenoic acid, the total content is around 90%, in which the hexadecanoic acid is the most, above 40%, next is the octadecenoic acid, around 30%.

结果表明：沙门氏菌属、埃希氏菌属、弧菌属和志贺氏菌属全细胞脂肪酸组分都含有十四烷酸、十六烷酸和十八碳烯酸，这三种脂肪酸占总脂肪酸含量的90%左右，含量最高的是十六烷酸，含量在40%以上，其次是十八碳烯酸，含量在30%左右。

Objective To construct the expression plasmid of human Annexin V. express it in E. coli and label Annexin V with (superscript 99m)Tc using hydrazino nicotinamido.

目的 构建人膜联蛋白V表达载体并诱导其表达，应用双功能螯合剂联肼尼克酰胺偶联后应用核素(上标 99m)Tc标记。

The Escherichia coli is the most common pathogenic bacilli both on the medicineand the clinical veterinarian, the pathogenic can cause young pig"s yellow diarrheaand white diarrhea,and pig"s hydropsy, and so on.

大肠杆菌是医学和兽医临床上最常见的一种病原菌，其致病性株可引起仔猪黄痢、白痢、猪水肿等病。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

The chimeric immunotoxin was expressed in E. coli with the yield of about 30% of the total bacterial protein.

型IL-4R由IL-4Rα链和IL-2Rγ链组成，主要存在于T，B淋巴细胞、单核细胞及血癌细胞表面。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

Day-old specific pathogen free chickens were inoculated intratracheally with lowly virulent E.coli and /or mildly pathogenic avian influenza virus.

SPF鸡经不同顺序、不同时间间隔人工感染MPAIV和E.coli173株(O4)后，对试验鸡的病死率和针对大肠杆菌不同抗原体液免疫应答水平等进行研究。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。