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By screening on plate, 64 strains of bacteria with antagonism to E.coli were isolated from Rabbit caecum.

本研究旨在从健康兔肠道内分离出一株对大肠杆菌有拮抗活性的菌株，并对其鉴定到属。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.

方法从生番木瓜中提取mRNA后进行反转录，得到木瓜凝乳蛋白酶的cDNA，PCR产物克隆到pET22-b载体中，重组质粒转化至大肠杆菌BL21，进行诱导表达及表达产物的检测。

Coli and the disruption of pcaH in strain NCIMB 10467 has lead to loss of its ability to catabolize protocatechuate.

而pcaH在NCIMB 10467菌株中的敲除则使该菌完全丧失了代谢原儿茶酸的能力。

Of special interest is a suigeroxide dismutase[7] from E.Coli which contains Mnat an unknown binding site and redox catalyses the disproportionation of O2 to yield O2 and H2O.

Of特殊利益是suigeroxide氧化物歧化酶[7从大肠杆菌]其中载锰在一个未知的结合部位和催化氧化还原的氧产生歧化O2和H2O。

Coli BL21(DE3) with the amount of 11.49% through thin layer chromatography scanning analysis. Cavia cobaya was immunized using the purified cytorrhyctes to produce the anti-serum. Through the determination of the optimum working conditions, the sensitivity test, the specificity test, repeatability test and sample simulation test, the indirect ELISA method was established, which is a broad-spectrum, rapid and specific to detect various of food-poisoning Vibrio simultaneously.

融合毒素蛋白包涵体经纯化，免疫豚鼠制备血清抗体，确定间接ELISA的最佳工作条件，并进行敏感性、特异性、重复性及模拟样品检测试验，建立了食物中毒性弧菌广谱、快速、特异的同步免疫学检测方法。

The supernatant of the recombinant E. coli by sonication could not only repress the pigment synthesize of Chromobacterium violaceum ATCC 12472, but also could attenuate the intensity of bioluminescence of V. harveyi VIB391 and V. harveyi BB886 by 68% and 53%, respectively.

将表达的重组菌体经超声波破碎后，取其上清液作用于紫色色杆菌ATCC 12472，使其受密度感应系统控制的紫色色素的合成受到抑制；又用上清液分别作用于哈维氏弧菌的发光菌株VIB391和BB886，可使其发光强度分别减弱68%和53%。

Objective To express the N-terminal fragment of human mast cell chymase in E.coli and prepare its polyclonal antibody from mouse.

目的：在大肠杆菌中表达人肥大细胞类糜蛋白酶N端片段，并制备其鼠源性多克隆抗体。

YIp5 plasmid which is replicable in E. coli but not in yeast is served as clonic carrier and ARS sequence in total DNA of rice cloned by air gun experiment.

以能在大肠杆菌复制，但不能在酵母菌中复制的YIp5质粒作为克隆载体，鸟枪法克隆了水稻总DNA中的ARS顺序。

The commonest organisms isolated in patients with SBP include Escherichia coli, gram positive cocci and enterococci.

在SBP患者分离出的最常见的微生物包括大肠埃希杆菌、革兰阳性球菌和肠球菌。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。