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A separated pathogenetic material of edema disease of pigling from endo-toxin is isolated, which is prepared from hemolytic E coli O139:K82 of the natural edema disease cases, by spreading culture, repeatedly freezing and thawing, rapidly centrifugalizing, concentrating and doing sephadexG-200 column chromatography.

本文主要报道从仔猪水肿病自然病例分离的溶血性大肠杆菌O_(130)：K_(82)，通过扩大培养，反复冻融，离心、浓缩，进行SephadexG-200柱层析，分离出了一种与内毒素分开的仔猪水肿病致病物质。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

Avian Escherichia coli ; type 1 pili ; structural gene ; cloning

鸡大肠杆菌； 1型菌毛；结构基因；克隆

The antibacterial effects of ethanol extract of Rhynchosia volubilis Lour roots on staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and pneumobacillus were observed.

方法（1）鹿藿醇提取物按照国际计划生育基金会认可的杀精子实验方法，对10只小鼠附睾精子悬液和10例人精液进行体外杀精实验。

The lowest inhibiting concentration of ethanol extract of Rhynchosia volubilis Lour roots on staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and pneumobacillus were 1.0,1.0,4.0,4.0 mg/ml,respectively.

结果鹿藿醇提取物对小鼠和人精子最低杀精浓度分别为50和100 mg/ml；对标准菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌MIC分别为1.0，1.0，4.0，4.0 mg/ml。

Resultsthe lowest spermicidal concentration of ethanol extract of rhynchosia volubilis lour roots on mouse and human sperm were 50mg/ml and 100mg/ml, respectively.the lowest inhibiting concentration of ethanol extract of rhynchosia volubilis lour roots on staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and pneumobacillus were 1.0,1.0,4.0,4.0 mg/ml,respectively.conclusionthe ethanol extract of rhynchosia volubilis lour roots has spermicidal and antibacterial effect.

结果鹿藿醇提取物对小鼠和人精子最低杀精浓度分别为50和100 mg/ml；对标准菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌mic分别为1.0，1.0，4.0，4.0 mg/ml。对临床分离菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌mic分别为0.5，2.0，8.0 mg/ml。结论鹿藿醇提取物对小鼠和人精子有一定杀精作用，对临床常见致病菌有比较强的抗菌作用。

Results: The antibacterials experiment showed that the usnic acid material and the SMEDDS had inhibitory action to staphylococcus aureus and pneumococcus, but no inhibitory action to pseudomonas aeruginosa and bacillus coli.

结果 松萝酸原料药及其自微乳制剂对金黄色葡萄球菌和肺炎球菌的生长有抑制作用，对绿脓杆菌和大肠杆菌无效。

The recombinant protein was purified with the different pH. Western blotting testing of the porcine expression of pGEMX POB in E.coli BL21 was positive.

利用非融合表达产品制备抗血清，检测融合表达的重组蛋白，Western-blot为阳性。

Pokeweed antiviral protein belongs to Ribosome-inactive Proteins. PAP has potent antiviral activity against many plant virus. pT1 was used as the template to amplify the deleted mutant PAP gene (N-deleted and N-,C-deIeted) by PCR and then the two genes were cloned into expression vector. Different IPTG-inducible expression vector containing different deleted mutant PAP gene was constructed and transferred into E.coli strain BL21 (DE3)-plysS.

美洲商陆抗病毒蛋白是一类能很好的抑制植物病毒的蛋白，很早就有报道低剂量的商陆叶片粗粉末就能明显的抑制TMV在菜豆叶片上局部枯斑的形成，PAP缓释剂也已用于病毒病的防治工作，但美洲商陆抗病毒蛋白属于核糖体失活蛋白，它对生长的细胞会有一定的毒性。

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48