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Sequence structure of a cloned gene is an important factor to determine its expression level in E.coli. In this paper, we studied the expression of a fragement of HIV-1 gp120 gene and a fragement of choler toxin A subunit gene in E.coli using a new expression strategyoptimizing the gene sequence as a whole.

外源基因的序列结构是影响外源基因在大肠杆菌中表达的重要因素，我们采用基于"外源基因全序列结构优化"的新的表达策略，研究了HIV-1 gp120-801和霍乱毒素A亚单位(CTA-246)基因在大肠杆菌中的表达情况。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Results Ganmaofang had definite bacteriostatic action on standard strain of staphylococcus aureus and escherichia coli, and clinical dissociative strain of staphylococcus aureus, escherichia coli, klebsislla pneumoniae, Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus simulans and beta streptococcus in vitro. Its MIC is between 0.075 g/mL and 0.2 g/mL. Ganmaofang could prolong the survival time of mouse infected by staphylococcus aureus and decrease the mortality of ill mouse, meanwhile it could enhance the survival rate of mouse infected by pneumoniae diplococcus.

结果感冒方体外对标准菌株金黄色葡萄球菌、大肠杆菌和临床分离菌株金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、表皮葡萄球菌、中葡萄球菌、溶葡萄球菌、乙型链球菌具有一定的抑菌作用，MIC 0.075~0.2 g/mL，体内可延长金黄色葡萄球菌感染小鼠的存活时间和减少感染小鼠的病死率，对肺炎双球菌感染小鼠可提高感染小鼠的存活率。

Coli HB101 expressed fimbriae as well as E. coli HB101 indicating the involvement of MrkF in fimbriation.

此外，基因重组大肠杆菌HB101与HB101皆能产生纤毛，显示MrkF可能参与纤毛组装过程。

Department of Microbiology and Immunology is primarily dedicated to teaching and research in protein secretion in Escherichia coli, cell wall synthesis and cell division in Escherichia coli, interactions and functions of proteins, mechanism of DNA replication arrest, microbial pathogenesis, microbial genetics, immunodeficiency, immunogenetics, infectious diseases, molecular biology, etc.

微生物学与免疫学系主要致力于微生物学与免疫学的教学与研究，其研究领域包括大肠杆菌的蛋白质分泌，大肠杆菌细胞壁合成和细胞分裂，蛋白质的相互作用和功能，DNA 复制抑制机理，微生物致病机理，微生物遗传学，免疫缺陷，遗传学，传染病，分子生物学等。

METHODS: Escherichia coli genomic DNA was extracted and purified from Escherichia coli 25922 by alkaline lysis method.

目的：探讨细菌DNA是否参与全身性炎症反应综合征的发生及其可能机制。

To evaluate the risk factor for Melanosis Coli. To investigate the relationship between Melanosis Coli and colorectal neoplasia.

目的 探讨结肠黑变病(melanosis coli， MC)的病因、发病特点，以及MC与结肠肿瘤、结肠腺瘤性息肉的关系。

Analysis of nuclear extracts of human cells has indicated a similar excision repair mechanism for nick-directed mismatch correction in higher cells, and several reconstituted systems that rely on purified human proteins have been described, such as hMutSα、hMutSβ、hMutLα. Recently, scientists discovered that nickel could be inhibitors of DNA repair. Nickel can greatly enhance the mutagenicity and genotoxicity of compounds in cigarette by inhibiting the nucleotide excision repair pathway in human cells. We have confirmed that nickel could inhibit MMR of E. coli and human and presumed that nickel inhibited repair before MutH incision of the MMR in E. coli. However, there is no evidence of mechanism of nickel inhibiting MMR in human cells.

镍是存在於香菸中的微量金属，近年来有学者发现镍是DNA修复反应的抑制剂，长期抽烟的人会容易因为香菸中化合物攻击DNA而引起突变，进而引起肺癌，而镍会抑制核苷酸移除修复系统（nucleotide excision repair；NER），所以使得体内的DNA损伤无法修复；过去实验室已经证实大肠杆菌的核酸配对错误修复系统会受到镍等重金属的抑制，并且推测出镍的抑制反应是抑制在修复反应的上游nicking的步骤；另外，也证实了人类的核酸配对错误修复系统也会受到镍的抑制，然而还没研究证实会抑制在核酸配对错误修复反应的哪个步骤。

The major route of β-alanine production for pantothenate in Escherichia coli is through the α-decarboxylation of L-aspartate, which is catalysed by the enzyme L-aspartate-α-decarboxylase. The panD mutant of E. coli is β-alanine auxotrophy.

在大肠杆菌中，用于泛酸生物合成的的β-丙氨酸主要是由L-天冬氨酸α位脱羧而产生，这个生化反应由panD基因编码的L-天冬氨酸-α-脱羧酶催化。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48