查询词典 coli-

With genomic DNA of Clostridium stercorarium as a template, a structural gene pel9A encoding pectate lyase was obtained by PCR amplification. The pel9A was cloned into pET28a to recombine an express plasmid pPel9A, and then the plasmid pPel9A was transformed into E. coli BL-21, expressed under the control of T7lac promoter at 37℃.

利用PCR技术从耐热梭状芽孢杆菌中扩增得到产耐热果胶裂解酶的结构基因pel9A，并将其克隆于表达载体pET28a中，最后将此重组质粒转化到受体菌E.coli BL-21中进行表达。

The E. coli ClpQY is an ATP-dependent protease that consists of an ATPase large subunit and a peptidase small subunit. The function of ClpQY protease is as a chaperon in refolding of misfolded peptides and as a protease to catalyse the degradation of abnormal proteins as well.

大肠杆菌ClpQY是一个ATP依赖的蛋白酶，ClpQY蛋白酶包含了具ATPase活性的大蛋白分子和一个小分子的peptidase，ClpQY蛋白酶作为一伴随蛋白质扮演分解错误摺叠的蛋白质，也为一催化分解不正常的蛋白质的蛋白酶角色。

By adding a sequence encoding a small peptide,〓, to the base sequence of pYZFcd20, a new phasmid pYZcpp3 was constructed. Using the pYZcpp3 to transform E. coli. 16c9, the genetically engineered bacteria 10916# was obtained. 10916# can secret the soluble chimeric antibody fragment anti-CD20 F〓 into periplasmic.

通过对质粒pYZFcd20的碱基序列的改造，在CH1基因的末端添加了一截二聚化结构的编码序列，实现了在大肠杆菌中直接表达可溶性抗CD20嵌和抗体片段F〓的目的。

Inan attempt to select more efficient biocatalysts ,the hydantoinase and carbamylase genes fromBurkholderia pickettii were cloned in Escherichia coli, achieve a co-experssed strains. The geneswere assembled to give the carbamylase gene preceding the hydantoinase gene. The recombinantstrains stably and constitutively produced the two enzymes and efficiently converted thecorresponding hydantoins onto p-hydroxyphenylglycine and phenylglycine .

我们试着寻找更快更有效的转化菌株，把来源于皮氏伯克霍尔德氏菌的表达该两种酶的两个基因同时克隆到大肠杆菌中，获得共表达两种酶的基因工程菌株，两个基因的顺序是 Dcase 基因在 Dhase 基因的前面，同一个启动子。

ABSTRACT Immobilized penicillin acylase (from E.coli) was used to catalyze 7 phenylacetamido 3 E propenyl cephalosporanic acid hydrolyzation into 7 amino 3 E propenyl cephalosporanic acid. Then, trans APRA was acylated with hydroxyethyl ester of 4 hydroxy D phenylglycine to obtain trans cefprozil.

以7 苯乙酰氨基 3 E 丙烯基 3 头孢菌素 4 羧酸为原料，在青霉素酰化酶作用下，首先酶法水解得到3 E 丙烯基 3 头孢菌素 4 羧酸，过滤固相酶，滤液调pH分离得到反式APRA固体；在青霉素酰化酶作用下，反式APRA再与对羟基苯甘氨酸乙二醇酯缩合，得到反式头孢丙烯；酶法合成所得的产品与进口反式头孢丙烯对照品一致。

E. Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠杆菌中分离多核苷酸■酸化酶的工作。

E.Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠桿菌中分离多核苷酸砱酸化酶的工作。

To study the effects E.coli purine nucleoside phosphorylase suicide gene on human gastric cancer MKN-45 cells as well as the bystander effect.

目的：探讨大肠杆菌嘌呤核苷磷酸化酶自杀基因对胃癌细胞MKN-45的抑制及杀伤作用，并证实旁观者效应。

In this paper, we studied the effect of sterilization on different bacteria and cyanobacteria in variant water sample of micro-electrolysis and high voltage electrostatic field, and the effect of physiological activity on E.coli and the characteristic optical absorption peak of phycocyanin of micro-electrolysis. The mechanism of sterilization and algae killing was approached.

本文研究了微电解、高压静电场作用对于不同水样中的不同种类的细菌和蓝藻的杀灭作用，以及微电解作用对于大肠杆菌的生理活性和对于蓝藻细胞内藻胆蛋白的光吸收特性的影响，探讨了杀菌灭藻的机理。

The expression of phycoerythrin beta and alfa subunit genes in E.coli and its activity study was done and will be done further.

另进行了龙须菜藻红蛋白b和a亚基基因在大肠杆菌体系中的蛋白质表达及蛋白活性研究。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48