查询词典 coli-

The result of specificity showed that different serotypes of strains were all positive for stx2 gene,while nonpathogenic E.coli ATCC23716 was negative.

特异度的测定结果表明不同血清型的大肠埃希菌均为stx2阳性，而非致病性大肠埃希菌ATCC23716则为阴性。

Objective: To clone the complete gene of nucleoprotein of H3N2 subtype avian influenza virus, and to express recombinant NP in E.coli for the function research.

目的：获得H3N2亚型禽流感病毒核蛋白全长基因，并在大肠杆菌中表达，以用于对NP功能的研究。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Antibacterial function of body wall was conducted by the following way: microorganism was directly resisted through the change of cuticle, such as incrassation of up-cuticle protuberance and increasing length of micro-floccus in the up-cuticle dissociation surface; exudation function of large granular orthochromatic mucous cells and reticulation mucous cells in the epidermis could be enhanced when the Eisenia fetida was induced by E. coli. Moreover, the mucus that contained compound substance of mucopolysaccharide and protein could make the bactericidal function or dilute the microorganism on the surface of earthworm, which could protect earthworm from invasion of microorganism; the content of neutral mucopolysaccharide of antibacterial function could be increased according with the exudation increment of small granular proteinaceous cells in the epidermis, which could resist bacteria invading into the epidermis of earthworm.

结果表明：蚯蚓的体壁主要是通过以下途径来抗细菌的侵袭：一是通过角质层的变化(如上胶层突起的增厚、上角层游离面中微绒毛的变长等)直接抵抗微生物的入侵；二是增加表皮中大颗粒粘液细胞和网状细胞的分泌功能，分泌出大量含有粘多糖蛋白液体复合物的粘液，在蚯蚓体表对细菌进行稀释或者起到直接杀菌的作用；三是通过增加小颗粒蛋白细胞的分泌作用，在蚯蚓的表皮中分泌出更多的抵抗疾病的中性粘多糖，从而抵抗进入蚯蚓体表内的细菌。

Coli can overproduce 38 000 antigen of Mycobacterium tuberculosis. Inclusion bodies are easy to purify and can protect the recombinant antigen from protease, on the other hand, it has not biological activity unless the denatured protein is accurately folded.

构建的大肠杆菌重组体能高效表达结核分支杆菌38 000蛋白质抗原，包涵体的表达形式有利于蛋白质纯化和蛋白质稳定，蛋白质必须经过正确折叠才具有生物学活性。

Escherichia coli 4-fluorotryptophan-substituted arginyl-tRNA synthetase was biosynthetically prepared and purified from a tryptophan auxotroph, which could overproduce this enzyme.

利用色氨酸营养缺陷型菌株在大肠杆菌精氨酰tRNA合成酶中成功地掺入了4-氟-色氨酸。

Coli. The ethanol Extract of Oxalis corniculata L had selective antimicrobial effect.

得到结论，酢浆草乙醇提取物具有选择性抑菌作用。

The team found that a bacterium called Mycobacterium paratuberculosis releases a molecule that prevents a type of white blood cell from killing E.coli bacteria found in the body.

该小组发现，一种称为结核杆菌的细菌杆菌释放的分子，防止一种白血细胞杀死大肠杆菌中发现的细菌在体内。

Pasteurization, which is often used for milk or waste milk, can be used to reduce bacteria levels and has been shown to be very effective at killing a variety of pathogenic bacteria including Salmonella, E. coli, Mycobacterium avium subspecies paratuberculosis, Mycobacterium californicum, Mycobacterium bovis, and Listeria monocytogenes.

巴氏灭菌常使用于牛奶或牛奶废料，它可以用来降低细菌水平，并已被证明能有效的杀死多种致病细菌，其中包括沙门氏菌、大肠杆菌、副结核分枝杆菌亚种、californicum分枝杆菌、牛分枝杆菌和李斯特菌。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48