查询词典 coli-

The profilin of Caryota mitis pollen was cloned and expressed successfully in E. coli BL21 (DE3), which will be used for further study on Caryota mitis pollen related allergy.

成功地克隆和表达了短穗鱼尾葵花粉profilin，为短穗鱼尾葵花粉过敏的诊断和免疫治疗奠定了基础。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

The prevalence of extended-spectrum betalactamase of Escherichia coli and Klebsilla spp. was 59.50% and 55.98%, respectively. 63.97% of Pseudo monas aeruginosa were isolated from sputum, drug-resistant rate of Pseudomonas aeruginosa in sputum from pa tients in ICU, neurosurgical department, respiratory and emergency pediatric department, respiratory department was 21.05%, 15.99%, 7.09% and 6.07% respectively.

大肠埃希菌和克雷伯菌属超广谱β-内酰胺酶产生率分别为59.50%和55.98%。63.97%的铜绿假单胞菌来源于痰标本，不同科室痰标本中分离的铜绿假单胞菌耐药率从高到低依次为重症监护室(21.05%)、神经外科(15.99%)、呼吸急救儿科(7.09%)、呼吸内科(6.07%)。

Moschus berezovskii IL-2 was cloned from Con-A stimulated peripheral blood mononuclear cells, and ligated with pIVID18-T vector, then transformed to Escherichia coli JM109. The positive recombinant was sequenced.

用林麝外周血单核细胞(peripheral blood mononuclear cells， PBMC)提取总RNA，RT-PCR扩增IL-2(interleukin-2， IL-2)，连接pMD18-T载体后转化大肠杆菌，筛选阳性克隆并测序。

Results The recombinant MPT 64 protein existed in inclusion bodies of E.coli.and amounted to 20%- 30% of totle cell protein.

具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。

Objective To explore the cell immunity of the 3rd stage larva of Musca domestica after being infected by Escherichia coli.

目的探讨家蝇3龄幼虫被大肠杆菌感染后的细胞免疫反应。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Coli BL21（DE3） which expressed Ag473-3His do not cross reaction with human neuroblast, such finding make this lipoprotein a potential vaccine candidate.

coli BL21（DE3）全菌菌体免疫产生的抗体并不会和人类神经原母细胞产生交叉反应，因此成为疫苗的首选。

The mRNA of CTGF, TGF-β1,ColⅠ and ColⅢ in neurofibroma tissue were over-expressed, whereas that in normal skin tissue was none or rare.

神经纤维瘤组织及瘢痕疙瘩组织中CTGF、TGF-β1、ColⅠ和ColⅢ的mRNA过表达，而正常皮肤组织无或仅有极弱表达。

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48