查询词典 coli-

The rewards are identical, however. Both Homo sapiens and E. coli are elite cosurvivors. And neither particularly has an advantage over the other in surviving the next million years. Actually, some pessimists give E. coli 100-to-1 odds on outliving humans, even though E. coli can currently live only in our guts.

事实上，在大肠杆菌和人类谁更长寿的赌局上，许多悲观主义者给出大肠杆菌100比1的赔率，尽管现在，这种微不足道的生物只能生存在我们人类的身体里。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Coli disease. And the use of allicin sulbactam or amoxicillin by a certain percentage for the Joint duck E.coli disease prevention observed experimental results, the results showed that: Allicin, amoxicillin, and sulbactam sodium single drug to duck the E. coli O0701 MIC were 1024ug/mL.Compound MIC its portfolio of 3～4.5ug/mL than single drug decreased by about 64 times.

利用大蒜素与阿莫西林或舒巴坦钠按一定的比例联合用于鸭大肠杆菌病的防治观察实验效果，结果显示：大蒜素、阿莫西林、与舒巴坦钠单药对鸭大肠杆菌 O0701的 MIC 均为1024ug/mL ，其复方组合 MIC 为3~4.5 ug/mL ，较单药降低了约64倍。

Results Causing the pathogen of the infection regard the Green's surname feminine gender rod bacteria as principle,occupying 90.2%,the escherichia coli is the most familiar to occupy 65% the next in order for transform the rod bactenia,viceescherichia coli,sewerage intestine rod bacteria,etc.of aerobacter aerogenes.

结果：引起感染的病原体以革兰阴性杆菌为主，占90.2%，大肠埃希菌最常见占65％，其次为变形杆菌、副大肠杆菌、阴沟肠杆菌、产气杆菌等。

Coll or MPAIV alone.the group inoculated with MPAIV and E.coli was more severe inflammation of the trachea, lung, and air sac and dispersion necrosis of parenchyma organs than that of groups inoculated with MPAIV or E.coli alone.

迅速，细菌数目也比A组多，l2 细菌数目差异显著，在3h后差异不显著。④各组脾脏。肾脏和肝脏在接种后偶尔可以分离到细菌，而且数量很少。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

Escherichia coli was first isolated in 1885 by the German bacteriologist, Theodor Escherich, as a normal inhabitant of the intestinal tract. Escherich named the organism Bacterium coli, the name reflecting the rod-shape of the cell (Bacterium means rod-shaped) and its intestinal habitat. The genus name Bacterium subsequently was changed to Escherichia in honor of its discoverer.

大肠杆菌于1885年首先被德国的细菌学家Theodor Escherich分离出来，因为它是一种普通的肠道居住者，Escherich把它命名为Bacterium coli，这个名字反映了细胞的杆状形态（Bacterium指的是杆形）和生活环境。

Results:The level of total anaerobic population and total aerobic population of excrement specimens of three kinds of IBS sufferers were different significantly, particularly the level of bifidobacterium and cillobacterium(P.01);the level of bacteroid population was higher significantly in diarrhea-predominant and mixed type of IBS sufferers (P.01);the level of enterococcus population was higher significantly in diarrhea-predominant IBS sufferers(P.05);among the aerobes, the number of Escherichia coli and Staphylococcus aureus colonies were increased by different level(P.01or0.05) in the IBS suffers, in particular, the number of Escherichia coli colonies in diarrhea-predominant IBS sufferers increased most significantly.

结果：三种类型的IBS患者粪便中总厌氧菌群和需氧菌群数目与健康人差异明显，特别是双歧杆菌属和乳酸杆菌属明显减少（P.01；拟杆菌菌落数在腹泻型和混合型IBS患者中明显增加（P.01）；肠球菌菌落数在腹泻型IBS患者中明显增加（P.05）；需氧菌中患者组大肠杆菌和金黄色葡萄球菌菌落数均不同程度增加（P.01或0.05），大肠杆菌菌落数在腹泻型IBS患者中增加最明显。

Objective:to study the level of total anaerobic population, total aerobic population, four kinds of anaerobes and two kinds of aerobes in excrement of sufferers of varies kinds of irritable bowel syndrome and that of normal persons.methods:excrement specimens from 52 ibs sufferers of different kinds and that of 18 normal volunteers were collected, and the level of four kinds of anaerobes and two kinds of aerobes were quantified.results:the level of total anaerobic population and total aerobic population of excrement specimens of three kinds of ibs sufferers were different significantly, particularly the level of bifidobacterium and cillobacterium(p.01);the level of bacteroid population was higher significantly in diarrhea-predominant and mixed type of ibs sufferers (p.01);the level of enterococcus population was higher significantly in diarrhea-predominant ibs sufferers(p.05);among the aerobes, the number of escherichia coli and staphylococcus aureus colonies were increased by different level(p.01or0.05) in the ibs suffers, in particular, the number of escherichia coli colonies in diarrhea-predominant ibs sufferers increased most significantly.conclusion:sufferers of different types of ibs have different intestinal tract dysbacteria phenotypes.

目的：了解不同类型肠易激综合征患者和健康状况正常的人粪便中总厌氧菌群和需氧菌群及常见的4种厌氧菌和2种需氧菌的含量情况。方法：对临床上确诊的各类型ibs患者共52例和18例健康状况正常的志愿者，采集粪便标本进行总厌氧菌群和需氧菌群及常见的4种厌氧菌和2种需氧菌定量检测。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48