查询词典 coli-

The regulatory elements of the tetracycline resistance operon of Eschenchia coli, Tet repressor and Tet operator, have served as the basis for the establishment of heterologous repression-based inducible system in many eukaryotes including yeast, plants, insects, mammals and parasitic protozoa, such as Trypanosomes, Entamoeba, Giardia, Toxoplasma, Leishmania and Trichomonas.

基于四环素抑制子(Tetracycline Repressor，TetR)和四环素操纵子(Tet Operator，TetO)相互作用的四环素可诱导转基因表达系统，已经广泛的应用于很多真核生物，包括酵母、植物、昆虫、哺乳类细胞和诸如锥虫、阿米巴、贾第虫、弓形虫、利什曼和毛滴虫的寄生原虫。

Coli. All points on the sphere are equidistant from the first life; therefore none is superior to the other.

。在这个球的表面上，所有点与表示最初生命的原点的距离都是相同的，因此，没有哪个物种比其他物种更高等。

The stable expression of exogenous gene in E.coli chromosome had no effect on the bacterial growth and propagation.

外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。

Coli strain stably and highly expressing recombinant Pseudomonas aeruginosa exotoxin A and for purification of expressed product.

目的 建立稳定、高效表达重组铜绿假单胞菌外毒素A的工程菌发酵及表达产物纯化工艺。

Coli＊s endotoxin or other exotoxin and reduce the incidence of diarrhea in livestock and poultry.

本品经肠胃消化吸收后，减少氮源的排泄，降低氨及硫化氢之臭味，促进环保。

MUG single tube quantitative assay method was introduced to Escherichia coli assay for bacterial suspensions,natural seawater,and fishery product samples.The detected data was analyzed and compared with multiple tube fermentation method.

采用MUG单管定量检测法对实验室制备的菌悬液、天然海水、水产品进行定量检测，并与大肠埃希氏菌多管发酵法的检测结果进行比对分析。

Then the flic gene was cloned into the E.coli expression vector pGEX-KG, which was induced with IPTG and the fusion protein was analyzed by SDS-PAGE and Western-blot.

构建重组表达质粒pGEX-flic，转化大肠杆菌工程菌BL21，经IPTG诱导表达后，通过SDS-PAGE及Western-blo对表达蛋白进行鉴定。

A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed by using 3 sets of primers that specifically amplify segments of the rfbE、fliC and eaeA genes. The target genes fragment of the PCR assay were 291 bp, 625 bp and 368 bp, respectively. Analysis of 30 strains demonstrated that this PCR system was specific.

以编码大肠杆菌O157抗原的rfbE基因、编码H7抗原的fliC基因以及编码毒力因子的eaeA基因为靶基因，选择3对引物，建立并优化了检测大肠杆菌O157：H7的多重PCR体系，扩增产物分别为291 bp、625 bp、368 bp，采用30株细菌验证了该多重PCR具有特异性。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Four vectors of pET vector system were chosen to express the enzyme in E. coli BL21(DE3). The main results were as follows: 1. The total RNA was obtained from fungus Syncephalastrum racemosum.

以总状共头霉菌株总RNA为模板，通过RT-PCR方法，获得了去除自身信号肽的天冬氨酸类蛋白酶syncephapepsin基因。2。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48