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Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon，邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高，为高产色氨酸基因工程菌的构建奠定了基础。

The complex can improve the antibiosis activity to Escherichia coli.

配合物能提高配体对大肠杆菌的抗菌活性。

Coli BL21, and to explore the antigenicity.

coli BL21中表达，研究其抗原性，为疫苗的开发奠定基础。

Expression of nitroreductase gene nor_1 in e.coli and the preparation of antiserum.

NOR1基因真核表达载体的构建及其对肝癌细胞生长的影响。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Finally, the fusion protein was expressed in E coli DH5α under induction by arabinose. And the fusion protein was 65 kD and account for 30% of the total protein of the bacteria.

SDS-PAGE电泳分析显示，融合蛋白分子量约65 kD，与预期蛋白分子量一致，并且目的蛋白占菌体蛋白的30%。

The structure and function of E. coli is often considered the archetype of all living organisms.

大肠杆菌的结构和功能常常被认为是所有生物的原型。

Method］ The antimicrobial activities of the ethanol extracts from 33 edible spice plants against Trichoderma viride, Aspergillum flavus, Aspergillum niger and Escherichia coli were studied in vitro using method of punching.

方法］以绿色木霉、黄曲霉、黑曲霉、大肠杆菌为供试菌，采用打孔法对33种香料植物的无水乙醇提取物进行体外抑菌试验。

Coli . The clones were verified by restriction enzyme digestion and PCR. Sequence and homology analyses showed that this phytoplasma strain shares high homology (99.0%) with the phytoplasma of Aster Yellows that belongs to 16SrI group.

通过酶切、PCR鉴定，对筛选得到的重组阳性克隆进行核酸序列测定及同源性比较分析，结果表明其与植原体16SrⅠ组中的西方翠菊黄化植原体同源率为99%。

Preparation of monoclonal antibody to human augmenter of liver regeneration : screening of hybridomas with unpurified antigen expressed by E coli .

人肝再生增强因子单克隆抗体的制备：以非纯化的大肠杆菌表达抗原筛选杂交瘤。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48