查询词典 coli-

The study of the effect of Glycerol on survival of the E.coli shew Glycerol could protect E.coli and improve dry, vacuum and implantational survival rate during ion beam implanting the E.coli. The whole survival rate of protected the E.coli implanted by 30keV N+ was 530-10000 times higher than unprotect ones. Glycerol changed bacteria outer color and survival rate curve. At the same doses, the color of the E.coli protected by five and one percent Glycerol was white or yellow, lighter than unprotected. In addition, the survival rate curve of the E.coli with Glycerol located above one without Glycerol, and the peak of which was higher and delayed at dose.

在研究甘油对低能离子注入E.coli存活的影响时，发现甘油保护可以提高E.coli的干燥存活率，真空存活率以及注入存活率，使得整个30keY氮离子注入后E.coli的最终存活率提高530-10000倍；甘油保护引起离子注入后的菌体颜色发生变化，在相同的剂量下，未用甘油保护的菌体颜色最深，1％甘油保护菌体颜色次之，5％甘油保护菌体颜色最浅；另外，甘油可以引起离子注入E.coli的"马鞍型"存活率曲线发生变化，甘油保护的E.coli存活曲线大致位于未用甘油保护的存活曲线的上面。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示，30～35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107／86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用，而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌，但可以很好地黏附表达于987P菌毛的大肠杆菌。

Three kinds of BCRC No.51534, 10322 and 10675 would be selected and acted as an experimental sample of Escherichia coli. Results shows that Escherichia coli of No.51534 will appear better performance because the maximum of open circuit voltage, closed current and power density are 1.01V, 22mA and 1342mW/m2, respectively. Concerning the effect of culture time with respect to different phase type on the electricity performance of MFCs, the time points on the intersection between lag phase and logarithmic phase, the middle of point of stationary phase for growth curve of Escherichia coli would appear a good performance of MFCs. In addition, the BCRC No. 51534 Escherichia coli possessing a better performance of MFCs than others would be suggested and applied to further studying. Comparison with the performance of MFCs with respect to electron mediator under different mole number, result shows that electron mediator of methylene blue with 4.63mM would appear a better electricity performance of MFCs than others. Concerning the different material of proton exchange membrane with PTFE-Nafion, Nafion 211, 212 and 117 with respect to the performance of MFCs, result shows that the Nafion 117 applied in MFCs will have a better performance of MFCs than other cases. Finally, the effect of molar concentration on the performance of MFCs would be expected at the studied cases of 0.4M, 0.2M, 0.1M and 0.05M respectively for cathode oxidant, result shows that a good performance of MFCs will happen at the condition of 0.2M. Those observations will be useful to improvement of MFCs in the further study.

於上述电池系统条件下，进行大肠杆菌生长曲线、电子传递介质、质子交换膜、电极与阴极氧化剂对电池电性效能分析；选择编号10322、10675与51534之大肠杆菌为实验菌株，依定量培养之生长曲线取出代表不同时生长特性时期的培养时间，利用亚甲基蓝作为电子传递介质进行实验分析从所测得的电量进行分析，以编号51534之大肠杆菌的微生物燃料电池有最大的开路电压为1.01V及最大闭路电流为22mA；当极化曲线中电压为0.47V、电流为11.4 mA时有最大的功率密度为1342 mW/m2；加以负载有平均工作功率密度294 mW/m2；从生长曲线与电性效能来分析，得知生长曲线的迟滞期与对数期的转变点与静止期的中间点有最佳电性效能表现；对於加入不同莫耳数之电子传递介质methylene blue、neutral red与thionine之电池效能表现，则以加入4.63mM methylene blue电子传递介质的电池有较佳平均功率密度230 mW/m2；另对於质子交换膜PTFE-Nafion、Nafion 211、Nafion 212与Nafion 117之电池效能表现，以Nafion 117质子交换膜的电池有较佳平均功率密度340 mW/m2；对於分析加入不同莫耳数浓度0.4M、0.2M、0.1M与0.05M的阴极氧化剂之电池效能，则以0.2M的阴极氧化剂的电池可得到较佳平均功率密度429 mW/m2。

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

临床分离及人工诱导大肠杆菌多重耐药株，氰氯苯腙结合荧光分光法检测不同耐药水平菌株对抗菌药物的摄入，确证主动外排系统的存在；构建了定量RT-PCR的内标准DNA，通过定量RT-PCR检测动物源性大肠杆菌AcrAB mRNA的水平；制备AcrA抗体，采用Western-Blotting检测不同耐药株AcrAB蛋白的表达水平；设计和构建AcrA、AcrB的Taqman探针，检测不同耐药菌株AcrAB的水平。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

An anaerobic-aerobic intermittent CSTR process was used to decolorize mixed dye with E. coli NO3 strain at a hydraulic retention time of 32, 18, and 10 h. The color-removal efficiency was around 70 ~ 80%. Under steady operation for over 117 h, the total decolorization was 10900, 18200, and 7590 for HRT=32, 18, and 10 h, respectively. After repeated operation cycles, the decolorization rate of E. coli NO3 was always higher than the original cycle. This may be attributed to the acclimation effect since the E. coli NO3 cells were repeatedly exposed to the azo dye.

NO3於HRT = 10、18、32小时之条件下进行厌氧-好氧间歇式CSTR生物反应器褪色操作，褪色率可以达到7080﹪左右；在约117小时的稳定褪色操作下，总褪色量分别为10900、18200及7590，这显示NO3在长时间之间歇式CSTR褪色操作下，不但稳定且具有优异之褪色能力；若将一组好氧、厌氧步骤视为一个循环去探讨褪色率之变化，则可观察到随著循环次数增加其褪色率逐次上升，之后则明显下降，这与摇瓶重复批次实验相似之结果，也可以解释为驯养效应所导致。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列，运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析，根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增，检测沙门氏菌和大肠杆菌Ⅰ类整合子；扩增出了相应基因片段并测序；61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子，Ⅰ类整合子的检测率分别为：81.97％和66.67％。

objective to study the risk factors on esbl producing strains of k.pneumoniae and e.coli.methods aprospective survey on esbl producing strainsof k.pneumoniae and e.coli for a36-month period.results the resistance rates ofe.coli were:98.08%for ctx,73.08%for amc.the resistance rates of k.pneuˉmonialwere;95.74%for ctx,97.87%for amc.the use rate of third generation cephalosporins was much higher than those of esbls nonproducing strains（p.05）.it induce esbls that3rd generation cephalosporins were used extensive.after strenghtening the antibiotic controls,esbls detection rate has gone down.conclusion the factors of infection of esbl-producing strains were the severity diseases,cellular immunological condition,improper medical manipulations.to prevent esbl-producing strains,reasonable antibiotics usage may be the effective measure.

目的 分析临床大肠埃希菌及肺炎克雷伯菌产esbls的危险因素，并加以控制。方法前瞻性监测产esbls菌的情况，并对感染者进行临床调查。结果产esbls的大肠埃希菌、肺炎克雷伯菌的耐药率头孢噻肟钠为98.08%和95.74%，阿莫西林+棒酸为73.08%和97.87%，产esbls菌感染者头孢第三代的使用率（70.21%）显著高于非产esbls菌感染者（39.47%）（p.05）；第三代头孢的大量使用诱导esbls的产生。通过加强抗感染药物的使用管理esbls检出率开始下降。结论严重的基础病、高龄、机体免疫力低下，长期住院者是esbls菌感染的易感宿主，皮质激素、化疗及介入性疗法是esbls感染的高危因素。滥用抗感染药是产生esbls的重要因素，合理使用抗感染药是防止esbls产生的主要措施。

Induced by 4℃ low temperature, Escherichia coli inoculated in water can enter the viable but nonculturable state; in the room temperature, Escherichia coli may keep culturable state for a long period; eutrophic culture media of TSA can produce more bacteria count compared with oligotrophic culture media of PCA; evaluating the bacteria count Escherichia coli in water by plate count method may underestimate the risk of intestinal pathogen contamination.

在4℃低温诱导下，大肠埃希菌在水中可进人活的非可培养状态；在室温下，大肠埃希菌在水中可保持较长时间的可培养性；富营养的TSA琼脂检测的菌落数多于寡营养的PCA琼脂；用平板涂布法检测水中的大肠埃希菌可能低估了肠道致病菌污染的风险。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。