查询词典 coli

Escherichia coli was first isolated in 1885 by the German bacteriologist, Theodor Escherich, as a normal inhabitant of the intestinal tract. Escherich named the organism Bacterium coli, the name reflecting the rod-shape of the cell (Bacterium means rod-shaped) and its intestinal habitat. The genus name Bacterium subsequently was changed to Escherichia in honor of its discoverer.

大肠杆菌于1885年首先被德国的细菌学家Theodor Escherich分离出来，因为它是一种普通的肠道居住者，Escherich把它命名为Bacterium coli，这个名字反映了细胞的杆状形态（Bacterium指的是杆形）和生活环境。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

To evaluate the risk factor for Melanosis Coli. To investigate the relationship between Melanosis Coli and colorectal neoplasia.

目的 探讨结肠黑变病(melanosis coli， MC)的病因、发病特点，以及MC与结肠肿瘤、结肠腺瘤性息肉的关系。

The study of the effect of Glycerol on survival of the E.coli shew Glycerol could protect E.coli and improve dry, vacuum and implantational survival rate during ion beam implanting the E.coli. The whole survival rate of protected the E.coli implanted by 30keV N+ was 530-10000 times higher than unprotect ones. Glycerol changed bacteria outer color and survival rate curve. At the same doses, the color of the E.coli protected by five and one percent Glycerol was white or yellow, lighter than unprotected. In addition, the survival rate curve of the E.coli with Glycerol located above one without Glycerol, and the peak of which was higher and delayed at dose.

在研究甘油对低能离子注入E.coli存活的影响时，发现甘油保护可以提高E.coli的干燥存活率，真空存活率以及注入存活率，使得整个30keY氮离子注入后E.coli的最终存活率提高530-10000倍；甘油保护引起离子注入后的菌体颜色发生变化，在相同的剂量下，未用甘油保护的菌体颜色最深，1％甘油保护菌体颜色次之，5％甘油保护菌体颜色最浅；另外，甘油可以引起离子注入E.coli的"马鞍型"存活率曲线发生变化，甘油保护的E.coli存活曲线大致位于未用甘油保护的存活曲线的上面。

Azoreductase in E.coli.JM-109 can damage -N=N- to decolorize azo dye. Decolonization conditions,such as dye categories,temperature,pH,salinity,oxygen were studied in this article.E.coli.

由于E。 coli JM-109含有的偶氮还原酶能破坏偶氮染料的-N=N-使其脱色，因此本文考察了其脱色的条件（温度，pH，盐度，供氧量）及其适用范围。

Coli. It will give a good advice for heterogenous expression in E.coli

它对于在 E。 coli 中异源蛋白的表达效率给出了很好的建议

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Finally, wetested its antibacterial activities by E.coli and S.aureus.Methods In this research, the recombinded gene was cloned into the secretion vector pTYB12 and expressed in the E.coli BL21DE3. We did the preliminary research on secretion-expression of the gene. During the purification procedure, we used the chintin beads to wash off the sundry protein, then dialysed the collecting liquid to get rid of the salinity and the other small molecular peptides.

将上述重组基因抗菌肽的氨基酸片段克隆并拼接到分泌表达载体pTYB12上转染大肠杆菌(E.coli BL21DE3)后进行分泌表达，破碎离心收集的诱导表达大肠杆菌，首次应用几丁质珠柱分离纯化系统进行分离纯化，分离洗脱杂蛋白后透析去除盐类物质及其它小分子肽，得到目的重组基因抗菌肽。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失