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The expression efficiency of codon-optimized amyFF was increased by 12 times in Escherichia coli.

其中的amyFF在大肠杆菌中表达量提高了12倍。

The upstream regulatory region of the storage protein was separated from Glycine max variety "Ji Nong T7018" genomic DNA, according to published glycinin 11S G7 subunit (Gy7) gene(GenBank AF319776) sequence, a pair of primers was desgeined, in order to amplify the array before Gy7 gene initiation codon.

我们以大豆品种&吉农T7018&的基因组DNA为模板，根据已发表11S球蛋白G7亚基(Gy7)基因(GenBankAF319776)序列，设计一对引物，通过PCR扩增得到Gy7基因的启动子序列。

Codon partition schemes were compared in phylogenetic analyses at subfamily level for Gyrinidae and at family level for Hydradephaga based on partial mitochondrial COⅠ gene sequences.

利用PAUP和MrBayes软件，对线粒体COⅠ基因序列3个密码子位置的数据模块分别进行了豉甲科和水生肉食亚目在亚科或科水平上的系统发育学分析，结果表明第二密码子数据模块获得了理想的分析结果。

The codon-optimised Sjc-97f3 can be successfully expressed in Pichia pastoris and the recombinant protein is highly immunogenic in mice.

密码子优化的Sjc-97f3在毕氏酵母中获得高效表达，其产物具有良好的免疫原性。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

Methods p53 codon 72 were genotyped with pcr-rflp technique for 156 zhuang healthily long-living people (aged 90～105, mean 93.2 years, longevous group) and 142 healthy controls (20～72, mean 34.8 years, control group) from bama area to analyze the polymorphic genotype frequencies.

应用pcr-rflp技术对巴马156例（年龄90～105岁，平均93.2岁，长寿组）健康壮族长寿老人和142例健康壮族成年人（年龄20～72岁，平均34.8岁，对照组）的p53基因进行分型，了解这些基因的多态性，分析该基因频率在长寿组和对照组之间的差异及其与长寿的关系。

Coding and non-coding sequences are expected to have very different patterns of sequence changes because of the following characteristics of coding sequences: biased codon composition, higher conservation, absence of stop codons and frameshifts, higher fraction of synonymous than nonsynonymous substitutions, higher frequency of substitutions corresponding to changes of chemically similar amino acids, higher proportion of mutable contexts.

我们可以预期可转录的序列及不可转录的序列会有相当不同的序列改变特徵，因为可转录的序列有以下特色：偏颇的密码子组成，较高的保存度，有终止密码子和移码的出现，相较於异义取代有较高比例的同义取代，较高的频率出现化学结构相似的胺基酸取代，较高比例的可变之前后序列。

All kinds of these study results, including analyzing the nucleotide composition, the amino acid composition, the nucleotide diversity, the synonymous and the nonsynonymous, the transition and the transvertion, the frequencies of the codon used, the construction (including four stems and four loops) of tRNAleu,the phylogeny,suggest that great distinctness present among the genera and small difference even none among species.

在各种分析中(包括核苷酸基本组成分析；氨基酸基本组成分析；核苷酸多样性分析；非同义替代和同义替代分析；密码子使用频率分析；转换与颠换分析；tRNAleu基因的四臂四环二级结构分析；&翻译启始&和&翻译终止&在进化过程中的变化；系统发育分析)，属间的差异相当明显，而种间差异很小甚至是没有任何不同之处。

Absence of association between HPV DNA, TP53 codon 72 polymorphism, and risk of oesophageal cancer

HPV16感染对食管癌及P16、P53基因表达的影响。中国实验诊断学 2004；

Objective To decide the base sequences of target position for gene therapy, the K-ras point mutation and its style at codon 12 in human pancreatic cancer cell line Patu 8988 was detected.

目的 检测人胰腺癌细胞株Patu 8988 K-ras基因点突变及其突变方式，明确基因治疗靶点的碱基序列。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。