查询词典 codon

The 5＇coding region of its eDNA was optimized according to the codon bias of Pichia pastoris to make it secretively express in Pichia pastoris with higher performance. Two-step DNA synthsis was used to synthesize the eDNA sequence being optimized of ADTZ. OPT-ADTZ was connected with constitutive plasmid pGAPZaA to construct the reecombinant plasmid pNOA. pNOA was linearized and then transformed into Pichia pastoris GS115. Then codonoptimized ADTZ was constitutively and secretively expressed in Pichia pastoris.

为了使重组蛋白rADTZ能在毕氏酵母中高效分泌表达，根据毕氏酵母密码子的偏好性对rADTZ的5'末端的编码区域进行了优化，利用two-stepDNAsynthsis技术合成出ADTZ优化的基因序列OPT-ADTZ，并与组成型表达载体pGAPZaA连接，构建重组质粒pNOA，线性化pNOA后转化至毕氏酵母GS115中，实现了密码子优化的rADTZ组成型分泌表达。

Results:(1) There were two types of HBsAg termination codon: TAA and TGA in 174 reference sequences.

结果：(1)在174例参考序列中，HBsAg的终止密码子共有TAA和TGA两种形式。

There is terminate codon TAA of the whole ORF at the 3D coding region termination.

在3D编码区末端有整个ORF的终止密码子TAA。

At the same time, it shows that the intervening sequence between transcriptional terminator T φ and translational stop codon TAA has no significant influence on TGF β1 expression.

同时发现，转录终止子Tφ与翻译终止密码子TAA之间的间隔序列对TGFβ1的表达没有明显影响。

RESULTS: There was a point mutation in the 12th codon in Patu8988 cell; the amino acid was mutated to valine.

结果：Patu8988胰腺癌细胞株12位密码子点突变为GGT→GTT，12位氨基酸突变为缬氨酸。

A key regulatory gene of TetR/AcrR family, acrR, was inactivated through the introduction of an internal stop codon resulting in overexpression of AcrAB that appears to be associated with ciprofloxacin resistance.

一个TetR/AcrR家族中调控基因acrR由于在其基因中产生了终止密码子而失去了活性，导致与环丙沙星抗性相关的基因AcrAB的过量表达。

High mutation rate of K-ras codon 12 wasobserved in gallbladder carcinoma(60%),bile ductcarcinoma(81.8),ampullary carcinoma(90%) and duodenalcarcinoma(70%).

在胆囊癌、胆管癌、壶腹癌及十二指肠乳头癌中均检测到了高比率的K-ras基因12密码子的点突变。

Results ①DNA sequencing showed that pBX1 contained a 477bp inserted gene fragment,and when it was compared with the published sequence of the specific region of the gene of the 83kd antigen protein from Borrelia burgdorferi B31 strain,only one amino acid codon was different.

结论该研究成功地对莱姆病螺旋体83kd抗原蛋白特异性区段进行了基因重组和表达，为进一步研究其在莱姆病血清学诊断和基因工程亚单位疫苗研制中的应用奠定了基础。

The nucleotide composition and the phylogenetic relationship among 14 species in Coreidae based on 412bp sequences of Cyt b gene were studied. The results showed: the average contents of T, C, A and G were 34.2 %、11.4 %、35.7 % and 18.7 %, respectively; The content of A+T (69.9 %) was obviously higher than that of G+C (30.1 %), and come up to 82.8% at the third position of codon.

以线粒体细胞色素b基因作为分子标记，首次对缘蝽科4亚科14种昆虫进行序列测定，获得Cytb基因412bp的序列片段，该片段中碱基T、C、A、G的平均含量分别为34.2%、11.4%、35.7 %和18.7 %，A+T平均含量为69.9 %，明显高于G+C含量（30.1 %）。

Three major clusters of numt sequences ( cyt b numt、 ATP8+ATP6 numt and ND4+ tRNA-His+ tRNA-Ser numt) were particularity amplified and sequenced from 10 species within Galliformes and A.galericalata. Comparison numts sequences with homologous mitochondrial partial genes get the different evolutionary characteristics and evolutionary speed compared to mtDNA, then examine the age of insertion of numts using the sequence divergence of numt from the mtDNA. Finally, joined Coturnix japonica、 Coturnix chinensis、 Lophura leucomelana、 Lophura swinhoii and A.albifrons mitochondrial sequences in GenBank, we study the importance function to phylogenetic research of mitochondrial genes and homologous numts.The evolutionary rate of Numts is lower than their authentic sequences of mtDNA, cyt b and ND4 gene evolve faster than homologous numts 2.5 times; ATP8+ATP6 gene evolve faster than numts 18.9 times in Galliformes. Numts show less codon position bias and transition bias under not phylogenetic pressure.

根据2004年3月GenBank登录的红原鸡核基因组草图与其线粒体基因组比对发现红原鸡核基因组中存在3段较长的线粒体假基因，利用PCR方法扩增鸡形目10种鸟类和雁形目中鸳鸯核基因组中3段numt序列：cyt b numt、ATP8及下游ATP6 numt和ND4+tRNA-His+tRNA-Ser numt，测序后分别比较线粒体基因与核同源numt序列的进化特点和进化速率，确定numt序列插入核基因组的时间，以及结合GenBank已登录的日本鹌鹑、蓝胸鹑、黑鹇、蓝鹇和1个雁形目物种——白鹅雁(A.albifrons)相应序列探讨两种同源序列对于鸟类系统进化发育重建的重要作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。