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METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞，加入EGM-2培养液，接种于包被人纤连蛋白的培养皿中贴壁培养，收集48h后的悬浮细胞重新贴壁培养至第7天，利用免疫荧光和流式细胞术鉴定。

The magnetite with a mean diameter of 8nm was prepared by chemical coprecipitation methods. By the addition of nonionized oleic acid to the suspension of magnetite in solution during precipitation, the magnetite nanoparticles were coated with a double surfactant layer. A gel-like precipitated precursor is then formed because of the hydrophobic interaction among those coated units. By transferring of this precursor to an organic solvent, the surfactant double layer on Fe〓O〓 nanoparticles reduces to one; however, it still remains as a double layer by transferring to an alkaline aqueous solution. Both these cases give in stable colloids of superparamagnetic Fe〓O〓 nanoparticles.

从解决无机磁性Fe〓O〓颗粒与有机烯类单体的相容性入手，采用化学共沉淀法，制备了平均直径为8nm的超顺磁性Fe〓O〓颗粒，在制备过程中以非离子形式引入油酸，在Fe〓O〓颗粒表面形成一个具有双层结构的疏水外壳，并通过疏水相互作用凝聚成磁性Fe〓O〓凝胶，能够以单分子层结构形式溶解在非极性溶剂中，也能够以双分子层结构形式溶解在碱性水溶液中，形成稳定的超顺磁性Fe〓O〓溶胶。

Ash composition and background gain as the non-coated material opacity and polyporous increases, GCR application may not suitable for all non-coated material users.

灰身分替代和底色裨益由于否涂布质料不透明性的贬矮和多孔性的增增，GCR搁置或者并不得当所有的否涂布质料用户。

Proximal bone stress transfer has been thought to be less in association with proximally coated stems as compared with extensively coated stems.

近端涂层的股骨柄与广泛涂层的股骨柄相比，近端骨压力传导被认为较小。

In order to enhance the miscibility between the inorganic and the organic phases,the silanol coupling agent is made from the slane VTMS and the colloid silica. Then the silanol coupling agent and nano-particles of ZnSb2O6 as the conductive filler are successfully bonded and the nano-particles of ZnSb2O6 will become more organic-like. The colloid silane-SiO2-ZnSb2O6 precursor solution is subsequently mixed with a homogeneous lsopropylalcohol solution of SR-399 acrylic monomers (Dipentaerythritol pentaacrylate) and the active free radical agent709. Under nitrogen purging, this reaction solution prepolymerize at 60℃. Then, the solution is spin-coated with the spin coator at room temperature. At last, the coated thin film is cured and crosslinked by the UV curing reactor to form the highly transparent thin film with good optoelectronic properties from hybrid organic-inorganic nano-composite.

首先以矽氧烷VTMS和colloid silica制备矽氧偶合剂，再以此偶合剂对分散於异丙醇溶液之奈米级的colloid ZnSb2O6做有机化改质，以增进其与有机高分子基材的键结相容性，我们将此colloid silane-SiO2 - ZnSb2O6前驱物与SR-399有机基材单体(Dipentaerythritol pentaacrylate)的异丙醇溶液与活性自由基光起始剂709均匀掺混后，在充满氮气反应系统下，加热至60℃进行预聚合反应，接著在室温下以旋转涂布机旋转涂布成厚度均匀薄膜，最后将此薄膜直接高温硬化或以UV硬化机网状键结硬化成透明与具部份导电性等光电特性之无机/有机奈米混成薄膜。

The method is as follows: n-butyl titanate, absolute ethyl alcohol, surfactant and concentrated hydrochloric acid are fully mixed and stirred for 4 to 6 hours under room temperature to obtain precursor solution, then the precursor solution is spinningly coated on a clean conductive glass substrate of an indium oxide tin coating, and then the conductive glass spinningly coated with the precursor solution is preserved for 25 to 72 hours at the temperature of between 20 and 30 DEG C and the relative humidity of between 50 and 75 percent below, and finally undergoes a high-temperature calcination at the temperature of between 350 and 700 DEG C.

将钛酸正丁酯，无水乙醇，表面活性剂和浓盐酸充分混合，在室温下搅拌4小时-6小时得到前驱剂，然后将前驱剂旋涂在清洁的氧化铟锡镀层的导电玻璃基底上，接着在20℃-30℃，相对湿度在50％-75％下，将旋涂有前驱剂的导电玻璃保存24小时-72小时，最后经过350℃-700℃高温煅烧。

The amount of Ni and Cr released from specimens coated with gold was much fewer than those from unplated specimens (P .05), which meant the stability of Ni-Cr alloy on the surface was improved. The biocompatibilities of gold-coated Ni-Cr alloy were better than the uncoated alloy, though there was no difference in biological ranking.

说明其耐电化学腐蚀能力明显提高；镀金后镍铬合金试件的镍离子、铬离子析出量降低，表面稳定性有了显著性提高；无氰镀金法镀金的镍铬合金生物相容性良好，从数据上比较优于未镀金镍铬合金，但生物学评级上无显著性差异。

We find that, during the course of coating, thermodynamic cortrol can influence efficiency and life for column more effectively than kinetic cortrol; the rinse and laying out after coated zwitterionic surfactant can also influence the thickness and uniformity of layer coated.

三。探索了 EIC 体系的实际应用在 EIC 体系的应用方面研究了无机、有机电解质和极性化合物在该体系的色谱保留行为，探索了一些无机、有机阴离子和极性有机分子在 EIC 体系的色谱分离。

Methods Eighty patients of open heart surgeries performed recently were divided randomly into two groups, group A:heparin coated artery filter, group B:non-heparin coated. Platelets, hemoglobin, haematocrit, and activated coagulation time were measured on 60 minutes of extracorporeal circulation and after extracorporeal circulation and ultrastructure of filter screen was observed after extracorporeal circulation by electron microscope.

80例体外循环心脏直视手术患者随机分为两组，每组40例。A组：用肝素涂层动脉过滤器，B组：用非肝素涂层动脉过滤器；并于不同的时间点观察血小板计数、血红蛋白、红细胞压积、激活凝血时间和过滤器内网在电子显微镜下的改变。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。