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RecommendationBySeason: Beef goulash broth served with garlic toast Spinach salad with warm bacon,grated egg and parmesan shave,coated with honey mustard dressing Beef madras cooked with pineapple, atop on jasmine rice and Num bread to finished Medium grilled duck breast, set on a cheese potato cake, glazed with a dark cherry sauce Pasta with Curried chicken pieces, zucchini and sweet corn, enrich

当季推荐：匈牙利牛肉汤配蒜香面包熏肉菠菜色拉配鸡蛋末，帕尔干酪及蜂蜜芥末汁印度浓汁牛肉咖喱配米饭及飞饼嫩烤鸭胸配奶酪土豆饼及黑樱桃汁奶油咖喱鸡肉酱，节瓜及甜玉米粒

Fresh Squid tentacle sections, mashed yellow croaker and pollution-free cubed carrot and green string bean sections are selected as raw materials which are then added, mixed and stirred with an adhesive, a seasoning agent and a tenderizer in proper amount to form fillings; the fillings are then moulded into caked filling cores which are steamed and cooled; the outer surface of the filling core is evenly coated with a layer of stiff paste, the outer surface of which is evenly attached with a layer of puffed outer coating so as to form a caked quick-frozen squid and vegetable steak which takes the shape of a puffed powder slice and is internally provided with a filling core wrapped with the stiff paste; after being quick-frozen, the squid and vegetable steak can then be made into the quick-frozen squid and vegetable steak food.

其是以新鲜的鱿鱼脚段、黄花鱼泥和无公害的胡萝卜丁、青刀豆段作为原料，加入适量的粘合剂、调味剂、嫩化剂搅拌调和成馅料，由该馅料模制的饼状馅芯经蒸制、冷却后，在该馅芯外表面均匀挂有一层浆料，所述的浆料外表面均匀粘有一层蓬松的外裹料，形成一饼状的、外表为蓬松的粉片状物、内面由浆料包裹馅芯的鱿鱼蔬菜排，该鱿鱼蔬菜排速冻后即成速冻鱿鱼蔬菜排食品。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞，加入EGM-2培养液，接种于包被人纤连蛋白的培养皿中贴壁培养，收集48h后的悬浮细胞重新贴壁培养至第7天，利用免疫荧光和流式细胞术鉴定。

The method is as follows: n-butyl titanate, absolute ethyl alcohol, surfactant and concentrated hydrochloric acid are fully mixed and stirred for 4 to 6 hours under room temperature to obtain precursor solution, then the precursor solution is spinningly coated on a clean conductive glass substrate of an indium oxide tin coating, and then the conductive glass spinningly coated with the precursor solution is preserved for 25 to 72 hours at the temperature of between 20 and 30 DEG C and the relative humidity of between 50 and 75 percent below, and finally undergoes a high-temperature calcination at the temperature of between 350 and 700 DEG C.

将钛酸正丁酯，无水乙醇，表面活性剂和浓盐酸充分混合，在室温下搅拌4小时-6小时得到前驱剂，然后将前驱剂旋涂在清洁的氧化铟锡镀层的导电玻璃基底上，接着在20℃-30℃，相对湿度在50％-75％下，将旋涂有前驱剂的导电玻璃保存24小时-72小时，最后经过350℃-700℃高温煅烧。

The amount of Ni and Cr released from specimens coated with gold was much fewer than those from unplated specimens (P .05), which meant the stability of Ni-Cr alloy on the surface was improved. The biocompatibilities of gold-coated Ni-Cr alloy were better than the uncoated alloy, though there was no difference in biological ranking.

说明其耐电化学腐蚀能力明显提高；镀金后镍铬合金试件的镍离子、铬离子析出量降低，表面稳定性有了显著性提高；无氰镀金法镀金的镍铬合金生物相容性良好，从数据上比较优于未镀金镍铬合金，但生物学评级上无显著性差异。

The results show:(1) After soybean seed is treated with seed coating, The length of lateral root anlage Initiating area obviously increases in contrast with control (seed which is not coated with agent is regarded as control; shortened form is CK).The order is from greatness to smallness in turn as follow: HK＞ND＞CK.(2) Initiating progress of lateral root anlage is quickened when seed is treated with seed coating. General trend is as follow: HK＞ND＞CK. By variance analysis, treatment and control has obvious difference.(3) Initating status of lateral root anlage is definite related with contents of endogenous hormone. Occurring of lateral root is as the result of some endogenous hormone corresponded and playing manysided role, especially IAA plays important role in the cause of inducing to occurring of lateral root anlage.(4) The use of seed coating may elevate the number of lateral root anlage occurring and shorten the time of lateral root anlage occurring, especially the effect of Chemical control seed coating is better.

结果表明：(1)种衣剂包衣后，侧根原基发生区长度明显比对照(不包衣的种子为对照，用CK表示)增加，从大到小依次为：HK＞ND＞CK；(2)种衣剂处理加快了侧根原基的发生进程，总的趋势是HK＞ND＞CK，经F检验表明，处理和对照差异极显著；(3)侧根原基的发生状况与侧根原基发生区内源激素的含量有一定的关系，侧根的发生是几种植物内源激素相互协调、综合作用的结果，其中生长素类物质在诱导侧根原基的发生过程中起主导作用；(4)应用种衣剂可以提高侧根原基发生的数量，缩短侧根原基的发生时间，其中以化控种衣剂效果最好。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

Treatment can be 1%-2% sodium bicarbonate solution, pre-cleaner mouth in the breast-feeding or nystatin (10 million units / ml) coated surface of the skin 3-4 times a day; or coated with 1% crystal violet solution surface of the skin 2-3 times a day.

治疗可以是1％-2％碳酸氢钠溶液，预清洗口哺乳或制霉菌素（1000万单位/毫升）涂在皮肤表面，每天3-4次，或用1％结晶紫溶液涂在皮肤表面，每日2-3次。

In comparison with the conventional method of coating on the tool surface, carbide powders were coated with boehmite sol when dispersed in the mixture of boehmite sol and ethanol. The coated powders were then dried, separated, and hot-pressed. The sol preparation techniques were optimized in experiments and the property of the sol was studied at the same time.

本文从提高硬质合金刀具的耐磨性、探索新型刀具涂层方法入手，突破了在刀具表面进行涂层的传统方法，提出了用溶胶－凝胶法在硬质合金碳化物粉末表面涂覆一薄层氧化铝陶瓷制成复合粉末，然后热压制备刀具材料的新方法，开发成功一类新型的陶瓷涂层硬质合金复合刀具材料。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。