查询词典 closer

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

As I got closer I could see the back of the little girl's dress. It was grotesquely shaped. I figured that was the reason people just passed by and made no effort to speak to her.

当我向她走近的时候，可以看到她的后背，不可思议的形状，这大概是人们从她面前经过却没人试着和她说话的原因吧。

On the roof, outside the grubby apartment and closer to the sky, more dreams are possible.

的屋顶上，外面肮脏的公寓和更接近天空，更多的梦想是可能的。

As he flew closer, he saw that the surrounding barbed-wire fence had been mangled in places and the guardhouse at the entrance had been slightly damaged.

当他飞得更近的时候，他看见环绕着的带刺铁丝网已经倒塌，入口处警卫室有稍微地损坏。

Thoreau wasn't actually much of an outdoorsman, and his cabin was closer to the centre of Concord ,, than to any true wilderness, but for many Americans he remains the archetype the natural philosopher guiltlessly living off the grid, a mile from his nearest neighbour.

梭罗实际上并不是什么户外生活者，他的小屋更靠近康科德的市中心，而非什么荒野。但很多美国人仍然把他当成一个原型，看成一个离群索居的自然哲学家，最近的邻居都在一英里之外。

Portrayed as a callous grab for resources that ignores the recent killing of Guinean opposition protesters by government troops, closer analysis of the situation reveals as much about foreign perceptions of China in Africa as its does about one of the continent's most significant economic relationships.

在详细的局势分析中，中国被描绘成为了资源而冷眼旁观政府军杀害几内亚反对派抗议者，该分析透露了外国对于中国进入非洲的看法，还谈及了中非经济关系——中国是非洲最重要的经济伙伴之一。

Even now, your telomeres may grow shorter with each cell division, burning down ever closer to the point of guttering out.

既使如此，您的telomeres也许增长短与每细胞分裂，烧毁离点人类较近。

Liu Zhenyu: I feel a such problem, such I seeing, the client carries it of may directional to this kind user those who stick is closer, it is gutty an idea that resembles be being carried at any time, what I say is a concept that makes a website, because of this word, I feel to resemble the word of new media, client end, still be the application of a kind of Internet, if this kind applies, but if having a kind of application, I hope it is in at any time and place any when find it, I feel this is both have a few distinction.

刘振宇：我觉得这样的一个问题，我是这样看的，客户端它可能对于这种定向用户的贴的更近，它有种像随时携带的一个概念，我说的是一个做网站的概念，因此的话，我觉得像新媒体的话，客户端，还是一种互联网的应用，这种应用的话，但有一种应用的话，我希望它随时随地在任何的时候找到它，我觉得这两者有一些区别。

"Personalized medicine has the potential to increase overall health costs because additional tests are needed to identify ideal patients, and manufacturing costs could be higher because there will be fewer batches that target smaller, selected populations," says Dr. Hageman."That said, patient outcomes will be expected to be much more successful with far fewer adverse effects, which brings us closer to our goal of eliminating deadly diseases and improving the overall quality of human life."

"个体差异化的药物可能会增加整个健康产业的成本，因为你需要为典型的病人做额外的药物实验；同时生产成本也会上升，因为针对每个特定的小部分人群都要生产小批次药物，"Hageman博士说："然而这一切的结果，是药物会使得病人会变得更好，带来更少的不利影响；这也将让我们更加接近我们目标：那就是消除绝症、提高人类的整体生活质量。"

I felt my mouth smile and he took a half a step closer to me.

我感到嘴在微笑，他靠近了半步。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。