查询词典 cloning

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

We propose that induction of T cell anergy may depend on the T cell growth, activation and differentiation state or cloning conditions.

结果提示，体内T细胞与克隆T细胞存在功能上的不同，体内T细胞的无能诱导可能需要不同的条件。

Projects included: Cloning and functional studies of diabetes related genes;Structural studies of thermostable Catechol 2,3-Dioxygenase by anomalous scattering; Prevention and cure, and influence factor studies of major child bearing blemish of national minority in Guizhou province.

该课题组的主要研究方向包括大规模高通量人类基因克隆及功能研究；基因芯片技术、生物信息学及功能基因组学研究；蛋白质晶体结构与功能；蛋白质热稳定机制；疾病相关基因的研究。

Cloning, expression and antigenic analysis of heat shock protein A gene of human Helicobacter pylori.

人幽门螺杆菌热休克蛋白A编码基因的克隆、表达及抗原性研究。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Objective: To study cloning expression of human arsenite resistance related gene.

目的 ：克隆并表达人类抗砷基因 human arsenite resistance related gene。

Cloning and Functional Analysis of mnh6 and mtp1 Genes in Rice Blast Fungus, Magnaporthe grisea Abstract Rice blast is a severe disease to harm rice and the rice blast fungus is a well-known ascomycete Magnaporthe grisea.

稻瘟病菌mnh6和mtp1基因的克隆和功能分析摘要稻瘟病是一种严重危害水稻的毁灭性病害，其病原菌为子囊菌Magnaporthe grisea。

The successful cloning technology, the birth of lives into the asexual reproduction of the times.

克隆技术的成功，使生命的诞生进入无性繁殖的时代。

Due to decay in the Asgard genome, a result of excessive cloning, they no longer possess the ability to reproduce sexually, and all Asgard that were still alive at the time of their self annihilation were clones .

由于衰变在 asgard 基因组中，因过度的克隆，因此他们也就不再具备能力复制性，以及所有 asgard 认为仍有生还在的时候，他们的自我湮灭被克隆。

So, if we can agree that we should ban human cloning, I am all for it.

因而，如果我们同意，我们就要禁止克隆人，我是全力支持的。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐