查询词典 cloning

Methods(1) Construction of plant expression vector containing RS geneThe cloning vector pT-RS was constructed containing RS gene extracted from the leaf tip of Vitis vinifera by PCR amplification and RS sequence was identified by PCR amplification,enzymes digestion and sequence analysis.The plant expression vector containing RS gene was obtained and RS sequence was identified by PCR amplification and double enzymes digestion too.

研究方法(1)含白藜芦醇合酶基因的植物表达载体的构建提取葡萄基因组总DNA，通过PCR扩增得到RS基因，将此基因连接到克隆载体pGEM-T Vector，得到重组载体pT-RS；经PCR、酶切及序列分析鉴定后，将RS基因克隆到植物表达载体pBI121，得到重组载体pBI-RS，用PCR及双酶切方法进行鉴定。

Cloning is most simply defined as the asexual reproduction of biological material.

谢谢。克隆最简单的定义是生物原料的无性繁殖。

Clone Cloning is the English transliteration simply, it is an artificially induced asexual reproduction.

克隆是英文clone的音译，简单讲就是一种人工诱导的无性繁殖方式。

Cloning of animals is a group of special animal groups, through human asexual reproduction means, From a background of animal cells is the same group of individuals.

克隆动物是一群特别的动物群体，是人类通过无性繁殖的手段，从一个动物细胞获得背景相同的个体群的过程。

Cloning and sequencing of the amplifications in 15 cases of Chinese blood donors and dialysis patients were carrited out.

用聚合酶链式反应扩增TTV DNA序列核苷酸1915到2185之间的片段，并将该片段克隆入pGEM-T Easy载体。

Study on Gene Cloning, Expression and Expression Profiling in Tissues of Polypeptide: N-acetylgalactosaminyl Transferase 2

多肽：N-乙酰氨基半乳糖转移酶2（pp-GalNAc-T2，E.C.2.4.1.4）的基因克隆、表达及组织表达谱研究

The availibility of the extracted TTV-DNA by the two methods, primers and PCR system was testified by cloning and sequencing the 3' and 5'NCR of patient 72#, who was TTV PCR- positive when used the DNA extracted by STG method. The sequence of 3' and 5'NCR showed 88% of analogy to that of Japanese isolate in the GenBank.

同时，说明用STG法提取的TTV DNA、设计的引物和所用的PCR系统可以有效地用于检测TTV，为今后利用PCR研究TTV的致病性等提供了条件。

In nurturing the process of Dolly, scientists used somatic cell cloning technology.

在培育多利羊的过程中，科学家采用了体细胞克隆技术。

Methylation profiling also poses an important topic for study of embryonic stem cell and somatic cell cloning.

此外，研究甲基化概况也在胚胎干细胞及复制动物的研究上引发一些重要的研究主题。

The cloning and sequence of figleaf gourd and squash give us a chance to study the relationship between GPAT and plant chill-resistance in molecular level, from a few different amino-acid residues compared with the two GPAT amino-acid sequences.

南瓜及黑子南瓜GPAT基因的克隆，可以使我们从二者推导的有限氨基酸的差异中对GPAT氨基酸组成及其与植物抗冷性作一定的探讨。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。