查询词典 cloning

Cloning and sequence analysis of pheromone receptor gene and pheromone precursor gene

信息素受体和信息素前体编码基因的克隆和序列分析

Cloning of DNA is made possible by the ability of bacterial plasmids and phages to reproduce after additional sequences of DNA have been incorporated into their genomes.

将一段额外的DNA序列整合到细胞质粒和噬菌体的基因组上进行繁殖，使DNA克隆已成为可能。

Cloning of insecticidal crystal protein gene and its expressing in E.

本研究从苏云金芽孢杆菌克隆得到野生型杀虫晶体蛋白基因，构建了用于细胞核或叶绿体转化的植物表达载体，并用激光微束穿刺法、土壤农杆菌介导法和基因枪法将杀虫蛋白基因导入油菜，获得了具有抗虫性的转基因植株。

So this, my view is that although the cloning technology is the wing flew over, but due to development the immature, so we should take him to examine the attitude of observation, and should not be blindly accepted.

那么对此，我的观点是，克隆虽然是科技一次重大飞越，但是由于发展上的不成熟，所以我们对他应该抱着观察审视的态度，而不应一味的接受。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

Directional cloning vectors contain a single-strand overhang on the 5' end and a blunt end on the 3' end.

定向克隆载体包含一条单链：带有突出端的5'端和带有平端的3'端。

Park, including research and development, the results show, seed multiplication and extension of processing four functional areas, the base will make full use of cloning technology to cultivate farming cattle, sheep, seeds, and deep processing.

园区包括研究开发、成果展示、良种繁育和延伸加工四大功能区，这一基地将充分利用克隆技术培育养殖牛、羊良种，并进行深加工。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

As one part of RGP in China,we focused our interests on cloning the geneswhich express specifically in rice flower and genes which express constitutelywith important function,and studying their structures and functions.

作为中国水稻基因组计划的一部分，我们小组的研究主要集中在克隆水稻花特异表达的基因以及一些具有重要生理意义的组成性表达的基因，并进一步分析其结构和功能。

Animal rights activists point out that cloning attempts often fail.

动物权利活动家指出，克隆尝试常常失败。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐