查询词典 cloning

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

Zonula occludens toxin ; gene cloning ; gene expression ; E.coli

封闭带毒素；克隆；表达；大肠杆菌

Branches, leaves and phloem of apple collected from an old orchard in Yanji, Xinjiang were used for total RNA isolation specific fragments of Apple fruit crinkle viroid were amplified by RT-PCR using the primers disigned according to the sequence AB104558 on GenBank. Ten sequences were obtained through recovering, cloning and sequencing, and also registered in GenBank accession No.

采集新疆焉耆地区的老果园中的苹果树枝条、叶片和韧皮部，提取总RNA，根据GenBank中的AB104558序列设计引物，利用RT-PCR技术扩增出苹果皱果类病毒（Apple fruit crinkle viroid ，AFCVd）特异条带，通过回收、克隆和测序，结果表明，获得的10条序列，已在GenBank中登录（登录号：EU031507～EU031516）。

Asexual animals like rotifers reproduce by cloning and this makes for a fixed gene pool.

无性生殖动物像蛭形轮虫一样通过复制繁衍，这会导致他们有一个固定的基因库。

The construct bearing 10 copies of ribozyme was attained through successively cloning four times.

通过连续4次克隆获得10拷贝核酶基因的载体。

In view of these facts, We tried to find something in the L-form induction, plasmid extraction and their function, and the cloning and expression of promotors from BCG in Bacillus subtilis. Our aim is to provide a basis for the expression of HBsAg gene in BCG and to add something to the molecuiar biology of mycobacteria.

正是有鉴于此，我们试图在分枝杆菌在液体培养基中的L型实验诱导、质粒的提取及其功能分析、卡介苗启动子在枯草杆菌的克隆和表达等方面有所发现，为利用卡介苗表达乙型肝炎表面抗原基因提供基础，也为我国分枝杆菌的分子生物学研究增加内容。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

The resulting patchwork of laws, people on all sides of the issue say,plicates a nationwide picture already clouded by scientific and ethnical questions over whether and how to restrict cloning or to ban it altogether.

在克隆问题上各方人士认为，关于是否限制克隆、如何限制克隆或索性禁止克隆的科学及伦理问题，已经使全国性立法变得扑朔迷离。

In genetic engineering, multiple identical copies of a gene are produced in cloning vectors, such as plasmids and phages.

在遗传工程上，相同的多拷贝基因是由质粒或噬菌体这样的克隆载体产生的。

World of new technology to Franchise as a major marketing models to the brand as the core to the interests of as a link to a win-win situation for the purpose to complete, mature marketing program based on the spirit of "sharing of benefits, risks with piculs, Casting brand, and sustainable support "League idea, wholeheartedly with every franchisee common" cloning "wealth.

世新科技把连锁加盟作为主要的市场推广模式，以品牌为核心，以利益为纽带，以双赢为目的，以完整、成熟的营销方案为依托，本着&利益共享、风险同担、铸造品牌、永续支持&的连盟理念，竭诚与每一位加盟商共同&克隆&财富。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐