查询词典 cloning

Eukaryon expression vectors containing humanbcl-2 and bax cDNA were respectively transfected into HCC-9204 cells,HCC cellsthat express Bax stably and highly were established,and an HCC cell strain thatexpress Bcl-2 at a 100% positive rate was obtained by cloning.

用脂质体介导的基因转染法分别将含有人bcl-2和bax cDNA的真核表达载体转染到HCC-9204细胞中，建立稳定高表达Bax的肝癌细胞，并用克隆化的方法建立100％稳定表达Bcl-2的肝癌细胞株。

Systems that support ex nihilo object creation allow new objects to be created from scratch without cloning from an existing prototype.

提供扩展 nihilo 对象创建的系统允许对象从空白中创建而无需从已有的原型中复制。

Years ago in a mysterious far Eastern country the first human cloning experiment took place.

50年前，在东方一个古老而神秘的国家中，第一次的克隆实验悄无声息的开展了。

The results of DGGE profile showed that the diversity of bacterial decreased with the fermentation process and Lactobacillus manihotivorans became the dominant species at the end of the fermentation. According to the results of sequencing and blasting of clones, only Weissella, Bacillus, Acetobacter and Lactobacillus were detected in two cloning libraries. And some genuses were found and not identified in the previous researches of liquor Daqu and fermented grains.

DGGE图谱分析显示，随着发酵时间的延长，酒醅中的微生物多样性不断降低；发酵结束时Lactobacillus manihotivorans成为最主要的优势菌种。16S rRNA克隆文库的测序结果在数据库中进行比对后表明，浓香型和芝麻香型酒醅中的微生物种属存在巨大差异，仅有Weissella、Bacillus、Acetobacter和Lactobacillus 4个属的微生物在两个文库中均能检测到，发现并鉴定出在白酒曲药和酒醅的研究中尚未报道的细菌属。

Somatic cloning by nuclear transfer bypasses the gonads and therefore is a good model to study imprinting regulations.

克隆动物作为印记基因研究的实验动物模型，已获得许多有意义的研究结果。

Some important issues, such as the identification of synonyms and homonyms, the classification of germplasm resources, the breeding with sexual cross and early selection, the discovery and evaluation of mutants, and even the localization and cloning of important genes are all important research subjects to be addressed.

例如，荔枝品种中同物异名与同名异物情况的区分、种质资源的鉴别与合理的分类、杂交育种与早期选择、突变的发掘与鉴定评价、甚至重要性状基因的定位与克隆等，都是很需要解决的研究问题。

Some of the great hopes for cloning include the ability to perform tra lants, whenever they are needed, using cloned orga .

人们对克隆的一些伟大希望包括在需要进行移植手术时，人们能使用克隆器官。

Moreover, gene cloning and heterogenous expression give a new approach to potential industrial utilization of low-adapted lipases.

而将低温脂肪酶基因在常温宿主中表达即基因工程菌的构建为这一问题的解决提供了新的思路。

DMY, isolated by positional cloning in medaka, was regarded as the first sex-determining gene in non-mammals.

从青鳉Y染色体定位克隆的DMY，被认为是第一个非哺乳类脊椎动物雄性性别决定基因。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。