查询词典 cloning

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Moreover, cloning itself isn't new; there have been many species that reproduced clonally and a few that still do.

再说，克隆本身并不陌生，曾有过许多无性繁殖的物种，还有少数物种依然如故。

Identification of susceptibility genes for complex diseases in animal model, here rat arthritis as an example, may include the following steps:①selecting inbred animal strains and establishing disease models;② segregating breeding in F2 animals and running linkage analysis to find QTLs;③ producing a series of congenic strains to narrow down QTL region under help of STR or SNPs markers;④ carrying out positional cloning of susceptibility genes, and ⑤ confirming the function of susceptibility genes.

以大鼠类风湿关节炎模型为例，定位克隆易感基因步骤包括：①选择亲代近交系，建立关节炎模型；②分离育种，连锁分析、确定数量性状位点；③建立Congenic系、窄化QTL区域；④位置克隆基因；⑤易感基因的功能验证。

Finally, the Complex Beast, Human Cloning, the gene transforms human, even the cors.

最后，合成兽、复制人、基因改造人、甚至兽人，这些幻想中的生物一一成为现实。

Before we assume that the market for human clones consists mainly of narcissists who think the world deserves more of them or neo-Nazis who dream of cloning Hitler or crackpots and mavericks and mischief-makers of all kinds, it is worth taking a tour of the marketplace.

我们总是一相情愿地假定克隆人的市场主要由那些认为世界上应该多几个自己的自大狂、梦想克隆希特勒的新纳粹或者是疯子、怪人、惹事鬼构成，但在下这个结论之前，我们很有必要先做一次克隆市场之旅。

Since the first identification and cloning of a SUT cDNA from spinach, many SUT cDNAs have been cloned and characterized in 34 different plant species, in both dicots and monocots.

自从菠菜中克隆到第一个SUT基因以来，已先后有多个SUT基因的cDNA得到克隆与功能分析，涉及34种双子叶与单子叶植物。

These include dictators using cloning to amass an army of "perfect soldiers" or wealthy egotists seeking to produce hundreds or thousands of copies of themselves.

这包括独裁者利用克隆技术聚集的&完美的士兵军&或寻求产生数百或数千份自己富裕的利己主义。

These include dictators using cloning to amass an army of " perfect soldiers " or wealthy egotists seeking to produce hundreds or thousands of copies of themselves .

这包括独裁者利用克隆技术招募一支由&完美的士兵&组成的精锐部队

These include dictators using cloning to amass an army of "perfect soldiers" or wealthy egotists seeking to produce hundreds or thousands of copies of themselves.

这包括独裁者利用克隆技术招募一支由&完美的士兵&组成的精锐部队；或者富裕的利己主义者复制上百或上千个自己。

These include dictators using cloning to amass an army of " perfect soldiers " or wealthy egotists seeking to produce hundreds or thousands of copies of themselves .

这包括独裁者利用克隆技术招募一支由&完美士兵&组成部队；或者富裕利己主义者复制上百或上千个自己。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐