查询词典 cloning

Result: Restriction enzyme analysis proved that cloning vector pEGFP-RECK was successfully constructed, and the sequencing results of it was in accord with expected. By transfecting the pEGFP-RECK vector into SHG44 cells, we could see the protein locate mainly in cell plasm.

结果：所得RECK基因融合蛋白表达载体序列与预期相符。pEGFP-RECK表达质粒转染SHG44细胞后，荧光信号主要集中在与细胞质有关的部位。

Adding ouiduet epithelial cell or granulose cell to CR1aa or TCM-199 co-culture, the cleavage rate, 4-6 cell developmental rate and 8-16 cell developmental aresignificantly increased (P＜0.05). We can conclude that: CR1aa and TCM-199 containing 5％FBS were the samewith somatic cell cloning in culture in vitro in Yanbian Yellow Cattle.

使用SPSS14.0软件对结果进行方差分析并多重比较后，综合考虑实验结果和方便操作时间与过程，得出以下结论：含5％FBS的CR1aa和TCM-199培养液适用于延边黄牛体细胞克隆胚胎的体外培养。

So cloning and marking the resistance gene of the downy mildew in cucumber are fundaments for disease resistance breeding of cucumber.

克隆和标记黄瓜霜霉病抗病基因，则是当前利用分子技术进行抗病育种工作的基础。

Objective To develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy-producing plant Jatropha curcas.

目的建立药用及能源植物小油桐非试管快繁技术。

In the 20th century,science has developed by leaps and bounds.although science is pushing human society forward ,its negative effect has resulted in many global problems ,the human are facing to environment pullution ,ecology unbalance ,agene technology and cloning man in the front of victory .which makes man confront the alternative of life or death.

20世纪，尤其是20世纪下半期，科学得到了更加迅速的发展，在科学推动人类社会进步的同时，它的负面效应也更突出并引发了全球问题，人类在胜利的面前又要面对全球性的环境污染、生态失衡、核威胁以及基因技术、&克隆人&等引发的冲击，使人类社会面临生死存亡的选择。

South Korean scientists say they have engineered four beagles that glow red using cloning techniques that could help develop cures for human diseases.

韩国科学家称他们运用克隆技术培育了四只发红光的猎狗，这项技术可以帮助治愈人类疾病。

When designing DNA cloning experiments, any possible biohazards associated with the creation of recombinations of genes must be contained, to minimize the potential risks to humans and to the environment.

在设计DNA克隆实验时，必须考虑到任何可能与基因的遗传因子重组有关的生物危害的产生，从而将对人类和环境的潜在危险减到最小程度。

Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年，Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作，包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

The monoclonal cells were selected using cloning rings. The expression of capsid protein was detected by indirect immunofluorescence and sandwich-ELISA. The empty capsids of FMDV were observed under electron microscope.

经间接免疫荧光和酶联免疫吸附测定（Enzyme-linked immunosorbent assay，ELISA）方法检测MDBK细胞中衣壳蛋白的表达，并在电镜下观察口蹄疫病毒空衣壳。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。