查询词典 cloning

Establishment of the murine tumor cell lines expressing human M-CSFR-m-M-CSF fusion gene: The syngenic BALB/c mouse myeloma derived cell line SP2/0 was transfected with pF. Four stable expression cell lines were obtained after selection using G418 and cloning by limiting dilution.

表达人M-CSFR-m-M-CSF的小鼠肿瘤细胞系的建立：以pF转染小鼠骨髓瘤细胞系SP2/0，应用G418筛选，经克隆化培养建立了四株稳定表达人M-CSFR-m-M-CSF的SP2/0细胞系。

The termini of PCR products and the misincorporation in routine PCR were studied by using UT-PCR, cloning and sequencing.

应用UT-PCR扩增、克隆等对PCR产物末端研究结果表明，PCR产物末端组成是复杂多样的，但主要是3'端突出一个A，占末端总数的67.3％；其次是平端，占末端总数的26.9％。

These provide the guarantee in experimental techniques for gene cloning and functional study of torenia by T-DNA tagging.

这为利用T-DNA标签进行蓝猪耳基因克隆和功能分析提供了实验技术上的保证。

And then,the soluble target protein was purified with Ni-NTA column,and its C-terminal was sequenced with mass spectrum analysis.13 amino acids of C-terminal is identical to the Plasmodium falciparum TCTP when blasted in GenBank. Plasmodium falciparum ; Translationally controlled tumor protein ; Cloning expressing

虽然报道认为TCTP具有钙结合、金属内环境稳定、细胞内信号传导、作为IgE依赖性组胺释放因子、作为青蒿素类药物受体蛋白(Bhisutthibhanet al。，1998)和在约氏恶性疟原虫青蒿类药物抗性系中过表达等功能，但其具体生物学功能尚待进一步研究阐明

Molecular cloning and disease association of hepatitis C virus: A transfusion transmissible agent.

范桂高。面临病毒性肝炎的医务人员的危险和预防。

Here we report the cloning, expression, purification and activity assay of Phenylalanyl-tRNA synthetase from Trypanosoma brucei in Escherichia coli host.

本研究用大肠杆菌成功克隆表达并纯化了布氏锥虫苯丙氨酰-tRNA合成酶并进行了活性测定。

Acknowledging the potential for his defeat, he also sent a single Spaarti cloning cylinder to his facility on Nirauan to produce a single, unaccelerated clone of himself.

在获悉自己有可能兵败身亡后，他将一个斯帕提克隆罐送至尼劳安，并为自己制造一个未经加速的克隆体。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明，T NF的两种形式在受体结合、生物学效应等方面各具特征［3，4］。M-TNF通过直接接触在组织局部中发挥效应的特性，有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

在克隆M-TNF cDNA的基础上，删除两型TNF转换酶酶解部位的编码序列，构建出mTNFm并测序验证；建立只表达膜型T N F的真核细胞株。

A kind of M-TNF recombin ant with a stable,uncleavable mutant typewas constructed for its gene therap y. Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明，T NF的两种形式在受体结合、生物学效应等方面各具特征［3，4］。M-TNF通过直接接触在组织局部中发挥效应的特性，有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐