查询词典 cloning

YAC is now prevailingly used as the first cloning vector for human genome analysis, but one problem that is not yet solved is the insert DNA fragment is not easily isolated from yeast chromosomes and vectors. So, we developed a new technique for the specific radioactive labelling of YAC insert by Alu-PCR technique.

因目前YAC中的插入片段还无法分离出来，因此我们对筛选到的这些YAC，用Alu-PCR技术将人基因组插入片段进行了特异扩增，并通过PCR条件的适当改变，获得较理想的标记效果。

The main objective of the dissertation was to improve and modify the experimental procedures of mouse nuclear transfer and pronuclear transplantation, respectively, and try to establish new possible methods for mouse cloning and pronuclear transplantation.

本论文的主要目的是改进和修改小鼠核移植和原核置换的实验程序，试图建立新的小鼠核移植和原核置换的可行方法。

On Plant Pathogeny and Cloning, Expression of Antibiotic Prote

的抑菌作用和抗菌蛋白基因TasA的克隆和表达论文标题芽孢杆菌（Bacillus sp。

Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit p112 gene.

发现了 3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因，进一步克隆、测序和比对分析初步表明：杂种特异表达的 cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和 2 个未知功能的新基因；杂种表达增强的 cDNA 片断为鸡蛋白酶体亚基 p112 基因。

In this thesis preliminary study of genes relevant to symboiosis of prototrophic revertant 10NH-1 was carried out by molecular cloning.

本文还对原养回复突变型菌株有关基因的克隆及其与共生作用的关系作了进一步研究。

CLONING OF THE AMERICAN MIND focuses on America's "illiteracy cartel" which, the author maintains, is built around an out-of-control psychographic consulting industry.

美心着重于克隆美国的&文盲卡特尔&，作者主张，建一个失控psychographic咨询业。

The successful cloning and expression of yak IL2 gene is putatively useful for the development of novel immunopotentiator to raise the immunity of yak against various infectious pathogens and the immuno-protective efficacy of vaccinations.

本实验成功克隆了犛牛IL2基因，其原核表达产物具有显著的免疫活性，这为研制新型免疫增强剂来提高犛牛对各种疾病的抵抗力，增强犛牛疫苗的免疫保护率奠定了基础。

Objective: To construct the prokaryotic expression vector of human cancer-testis gene NY-ESO-1 by the method of LP Recco PCR Cloning.

目的：利用PCR产物靶向克隆法构建人癌－睾丸抗原NY-ESO-1的原核表达载体。

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。