查询词典 cloning

For example, the generation of penda reproduced by using cloning might be weaker than the father's generation.

比如，用克隆技术繁殖出来的熊猫后代体质上可能不如前一代，容易患病。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

Currently, neither cloning S and Z gene nor identifying their products was successful. Phalaris coerulescens can be used as a model plant to study SI in the Poaceae. Segregating population and comparative genomics strategy were used to map S and Z, constructing high-resolution mapping of the target loci so that map base clone S and Z genes. It is a research orientation and hot spot of self-incompatibility in Poaceae.

目前S、Z基因的克隆和编码产物的鉴定均未有成功的报道，天蓝虉草可作为禾本自交不亲和性研究的一个模式作物，运用分离群体和比较基因组学方法构建S与Z位点的精细连锁图谱，以对自交不亲和基因进行图位克隆，是当前禾本科植物自交不亲和性研究的一个方向和热点。

This paper intends to give an overview of the production and catalytic mechanism of trypsin, the biochemical characteristics, gene cloning, ontogeny, and expression and regulation of piscine trypsins.

本文综述了胰蛋白酶生成、催化机制以及鱼类胰蛋白酶的生化特征、基因克隆、个体发生及表达调控等。

Cloning of cytosolic (GS1) cDNA and chloroplast glutamine synthetase (CS2) cDNA from Pisum satium was carried out previously in our laboratory.

谷氨酰胺合成酶(Glutamine synthetase， GS， E。 C。 6。 3。 1。 2)是植物氨同化过程中的关键酶，对植物的氮素吸收和代谢起着至关重要的作用。

Cloning of cytosolic (GS1) cDNA and chloroplast glutamine synthetase (GS2) cDNA from Pisum satium was carried out previously in our laboratory.

谷氨酰胺合成酶(Glutamine synthetase，GS，E.C.6.3.1.2)是植物氨同化过程中的关键酶，对植物的氮素吸收和代谢起着至关重要的作用。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

Therefore cloning VP7 genes and the certification of its transformation, expression, identification in Schizosaccharomyces pombe have great significance for further study of VP7 protein expression in plants and the development of nuclear PRV genetic engineering vaccines.

因此对该基因的克隆及其在酵母菌中的转化、表达、鉴定，对于进一步研究VP7蛋白在植物中的表达及研制PRV基因工程疫苗都具有重要意义。

Patients with clinical virologic breakthrough during NA therapy and 4 negative control patients without virologic breakthrough were selected for the study of quasispecies dynamics. All the 70 serum samples from these 10 patients were analyzed with the method of'PCR-cloning-sequencing'. Totally over 2,100 clones were sequenced. The target sequences obtained were aligned, compared and analyzed to study the mechanism of NA drug resistance and to understand the clinical course of NA therapy from a viral populational genetic point of view.

我们进一步采用国际认可的&金标准&&PCR-克隆-测序-序列分析&的方法，对6例发生临床病毒学突破的患者以及4例未发生临床病毒学突破的患者的全程血清样本进行了准种检测，共测序2100余个，以系统深入地对LAM和ETV治疗过程中HBV种群的演变及其临床意义进行探讨。

Result］Sox9 cloning and expression vector construction was achieved.Sox9 and FGFR-3 expression was identified in marrow stroma cells after transfection,whose proliferation activity was similar to precartilaginous stem cells.

结果］成功的完成了Sox9的扩增和表达载体的构建，重组载体转染骨髓基质细胞后能检测到Sox9、FGFR3的表达，增殖活性与骨骺干细胞无异。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。