查询词典 cloning

Based on the cloning of Spidroin2 cDNA of Nephila clavipes (Genbank Accession No. AF441245), we constructed a prokaryotic expression vector pET-28b-Sp by double digestion.

本研究在获得生长于中国的Nephila clavipes蜘蛛牵引丝蛋白Spidroin2 cDNA（Genbank Accession No。

The number of molecular markers for background selection in the development of NILs and QTL cloning were also discussed.

另外，本文还就分子标记辅助近等基因系选育中背景选择标记数的确定、基于QTL-NILs的基因克隆等进行了讨论。

By using the specific PCR primers and T-A cloning, two species of AOB, 1 species of NOB and 11 species of ANAMMOX were detected in the SBBR one-step completely nitrogen removal process. One of the AOBs dominated at this reactor belonged to the family of Nitrosomonas for the similarity of 98% while another one was almost the same with an uncultured one.

同时通过T-A克隆的方法，在系统中发现2类不同的AOB序列，一类与氨氧化菌标准菌株Nitrosomonas有98%的较高相似性，另一类则与其它一种未得到纯培养的AOB接近，相似性为99%。

The possible benefits of cloning,however,are numberous,for artificially producing human organs for transplant,and for preserving endangered animal species.

但是，克隆所能带来的可能的好处还是很多的，比如说：人工制造可用于移植的人体器官；保护那些濒临灭绝的动物种族。

Our strategy was constructing the cDNA expression library of the Oncosphere development stage by keeping away from the genome of Teania solium, and screening, cloning the immunogen genes, as well as expressing and analysis the interesting gene.

本研究避开庞大的基因组DNA，以构建六钩蚴发育阶段的cDNA表达文库为切入点，从中筛选、克隆免疫原基因，并进行表达和功能分析。

To overview the recent advances and problems in this area in this article, and put forth a new concept of reverse cloning technique based on ooplasmic transfer.

本文回顾了该领域的研究进展和存在的问题，并以此为基础提出&逆向克隆技术&这一新概念。

Therefore the concept of reverse cloning technique was proposed based on ooplasmic transfer.

以卵胞质转移为基础的&逆向克隆技术&是否能达到克隆动物的结果前景喜人。

According to the sequence and functional analysis result some ORFs was selected to overexpress in E. coli. Cloning of the endogenous plasmid SLP3 of S.

随后根据测序的结果以及复制区和接合转移功能区鉴定的结果，选择对一些重要的功能蛋白进行在大肠杆菌中的过量表达。

We report here the cloning and characterization of a novel human PanK gene.

从我们的大规模cDNA克隆和测序计划中，利用表达谱基因芯片筛选的结果并通过生物信息学分析，我们选取了4条基因进行进一步的研究，以探讨这些基因是否与疾病相关。

From his 2cramped laboratory in Lexington, Kentucky, doctor Panos Zavos claims he is on the 3brink of cloning a human being.

在肯塔基州列克星顿拥挤的实验室里，帕诺斯·扎沃斯医生声称他马上就要克隆人了。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。