查询词典 cloning

The present study has built a solid foundation for identification, characterization, gene cloning and standardization of relation proteins in the embryogenic tissues of Larix leptolepis.

从本研究所获得的具高分辨率日本落叶松胚性愈伤组织蛋白质图谱，将为今后与愈伤组织的分化能力相关的蛋白质的检测、分离和基因克隆，以及胚性能力相关蛋白标准化的制定等提供参考。

The commission also cautioned against preempting the use of cloning technology for purposes unrelated to producing a liveborn child.

该委员会还警告不要抢占利用克隆技术的目的无关的生产活的孩子。

We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.

从杂草赛葵上采集了2组病毒样本Fz1和Fs1，用双生病毒通用引物PA、PB对杂草样本进行特异性的PCR检测，结果发现：赛葵样本Fz1和Fs1都出现约500bp的条带，说明这些杂草赛葵上存在双生病毒。

The partial gene encoded the conserved region of PPO cloning was carried out in 6 species, including Ulva pertusa, Ishige okamurai, Sargassum fusiforme, Mangifera indica, Musa nana, Shiitakae lentinus, Pleurotus sapidus.

发现从低等的真核生物真菌中的香菇和紫孢侧耳，至较高进化的藻类铁钉菜和孔石莼，到高等绿色植物芒果和香蕉，均能够一次性克隆获得PPO的基因，这同时也说明包括海藻在内，PPO基因在生物中是广泛存在的。

Calyciforus in Donghu Lake. The reproductive pattern of parthenogenesis provides the species cloning matrilineally, and keeping the most parental mutations.

东湖作为一个中型湖泊，其环境条件的季节变化是可以预测的，因此萼花臂尾轮虫休眠卵的产生是有一定规律的。

Cloning of murine mem-brane-type-1-matrix metalloproteinase（MT-1-MMP）and its metanephric developmental regulation with respect to MMP-2and its inhibitor ［J］.Kidney International,1998;54:131-142.［3］Chamers AF,Matrisian LM.Changing views of the role of ma-trix metalloproteinases in metastasis ［J］.

MMP-2在癌组织中强阳性表达率及阳性表达率与卵巢癌的病理分级均无关，但有淋巴结转移的癌组织MMP-2强阳性表达率明显高于无淋巴结转移组（P.05），而MMP-2的阳性表达率高低与有无淋巴结转移关系不明显。

Method］ With filter paper as the sole carbon source, a Myxobacteria CMC0606 was obtained; through observing its morphology, 16S rDNA cloning and sequencing, the bacteria was identified; adding XAD-16 into fermentation broth, crude extracts of fermentation products were obtained.

方法］以滤纸为唯一碳源，得到1株粘细菌CMC0606；通过观察其形态及16S rDNA克隆与测序进行菌种鉴定；向其发酵液中加入大孔吸附树脂XAD-16，收获发酵产物粗提物。

Based on the cloning of Spidroin2 cDNA of Nephila clavipes (Genbank Accession No. AF441245), we constructed a prokaryotic expression vector pET-28b-Sp by double digestion.

本研究在获得生长于中国的Nephila clavipes蜘蛛牵引丝蛋白Spidroin2 cDNA（Genbank Accession No。

The number of molecular markers for background selection in the development of NILs and QTL cloning were also discussed.

另外，本文还就分子标记辅助近等基因系选育中背景选择标记数的确定、基于QTL-NILs的基因克隆等进行了讨论。

By using the specific PCR primers and T-A cloning, two species of AOB, 1 species of NOB and 11 species of ANAMMOX were detected in the SBBR one-step completely nitrogen removal process. One of the AOBs dominated at this reactor belonged to the family of Nitrosomonas for the similarity of 98% while another one was almost the same with an uncultured one.

同时通过T-A克隆的方法，在系统中发现2类不同的AOB序列，一类与氨氧化菌标准菌株Nitrosomonas有98%的较高相似性，另一类则与其它一种未得到纯培养的AOB接近，相似性为99%。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。