查询词典 cloning

The near isogenic lines, Ps and Pf, were developed by anther, which serves as materials for isolating and cloning Rf3 gene by using of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) we had improved in this study.

该特异蛋白质点的出现与消失可能与玉米CMS-S的育性恢复有一定关系，对其纯化分离将为Rf3基因的克隆奠定基础和进一步了解玉米质核互作不育表达模式的分子机制。

Near isogenic lines were the best populations for QTL fine mapping and positional cloning,A few studies had been reported their results on materials with improvement traits using

本文从QTL的定位群体，定位方法，研究现状，精细定位与克隆，以及QTL利用等方面对作物数量性状基因的研究进行了综述。

Representational difference analysis of cDNA is a powerful technique for cloning the differentially expressed genes between samples. Kandelia candel and Dunaliella salina are two important halophytes.

cDNA RDA技术是目前应用较为广泛的一种分离差异表达基因的方法，盐藻和秋茄是两种重要的盐生植物。

The present study has built a solid foundation for identification, characterization, gene cloning and standardization of relation proteins in the embryogenic tissues of Larix leptolepis.

从本研究所获得的具高分辨率日本落叶松胚性愈伤组织蛋白质图谱，将为今后与愈伤组织的分化能力相关的蛋白质的检测、分离和基因克隆，以及胚性能力相关蛋白标准化的制定等提供参考。

The commission also cautioned against preempting the use of cloning technology for purposes unrelated to producing a liveborn child.

该委员会还警告不要抢占利用克隆技术的目的无关的生产活的孩子。

We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.

从杂草赛葵上采集了2组病毒样本Fz1和Fs1，用双生病毒通用引物PA、PB对杂草样本进行特异性的PCR检测，结果发现：赛葵样本Fz1和Fs1都出现约500bp的条带，说明这些杂草赛葵上存在双生病毒。

The partial gene encoded the conserved region of PPO cloning was carried out in 6 species, including Ulva pertusa, Ishige okamurai, Sargassum fusiforme, Mangifera indica, Musa nana, Shiitakae lentinus, Pleurotus sapidus.

发现从低等的真核生物真菌中的香菇和紫孢侧耳，至较高进化的藻类铁钉菜和孔石莼，到高等绿色植物芒果和香蕉，均能够一次性克隆获得PPO的基因，这同时也说明包括海藻在内，PPO基因在生物中是广泛存在的。

Calyciforus in Donghu Lake. The reproductive pattern of parthenogenesis provides the species cloning matrilineally, and keeping the most parental mutations.

东湖作为一个中型湖泊，其环境条件的季节变化是可以预测的，因此萼花臂尾轮虫休眠卵的产生是有一定规律的。

Cloning of murine mem-brane-type-1-matrix metalloproteinase（MT-1-MMP）and its metanephric developmental regulation with respect to MMP-2and its inhibitor ［J］.Kidney International,1998;54:131-142.［3］Chamers AF,Matrisian LM.Changing views of the role of ma-trix metalloproteinases in metastasis ［J］.

MMP-2在癌组织中强阳性表达率及阳性表达率与卵巢癌的病理分级均无关，但有淋巴结转移的癌组织MMP-2强阳性表达率明显高于无淋巴结转移组（P.05），而MMP-2的阳性表达率高低与有无淋巴结转移关系不明显。

Method］ With filter paper as the sole carbon source, a Myxobacteria CMC0606 was obtained; through observing its morphology, 16S rDNA cloning and sequencing, the bacteria was identified; adding XAD-16 into fermentation broth, crude extracts of fermentation products were obtained.

方法］以滤纸为唯一碳源，得到1株粘细菌CMC0606；通过观察其形态及16S rDNA克隆与测序进行菌种鉴定；向其发酵液中加入大孔吸附树脂XAD-16，收获发酵产物粗提物。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。