查询词典 cloning

Homologic sequences; Resistant candidate genes; Disease resistance genes; Cloning

吕香玲；卢峰；宋波同源序列；抗病候选基因；抗病基因；克隆

Some scientists want to a ly the methods used in cloning animals to produce skin, orga , and other body parts for huma .

一些科学家想用克隆动物的方法，来为人类制造人类使用的皮肤、器官与其它的身体构造。

According to the successful experiences on transgenic animal production and animal cloning, gene targeting could be utilized in many fields including theory and application research on genomic modification (such as site-specific gene repair, genetic defect therapy, gene knockout, infaust gene modification, even the site-specific integration), improving expression level of specific gene in animal mammary gland bioreactors, and promoting the industrialization of transgenic animal production.

结合现有转基因动物和动物克隆技术方面的经验，利用基因打靶技术，开展动物基因组修饰的理论和应用研究，即可定点进行基因修复、治疗遗传缺陷、基因敲除失活不利基因，还可最终解决动物乳腺反应器存在的随机整合率高而表达率较低的问题，实现定点整合及乳腺特异表达，为转基因动物的产业化生产创造条件，代表了当今动物遗传学发展的主流方向。

Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen,thereby facilitating the cloning of the mutated gene.

插入突变是一种通过外源DNA整合的方式来获得突变体，并克隆得到对应突变基因的方法。

Total RNA was extracted from venom cells from Gloydius intermedius, FLE gene was amplified by RT-PCR. Then it was connected to pMD18-T vector by TA cloning. At last, sequencing and analysis the genesequence.

从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

Methods:Total RNA was extracted from venom cells from Gloydius intermedius,FLE gene was amplified by RT-PCR.Then it was connected to pMD18-T vector by TA cloning.At last,sequencing and analysis the gene sequence.

材料及方法：从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

Near isogenic lines were the best populations for QTL fine mapping and positional cloning,A few studies had been reported their results o...

本文从QTL的定位群体，定位方法，研究现状，精细定位与克隆，以及QTL利用等方面对作物数量性状基因的研究进行了综述。

The near isogenic lines, Ps and Pf, were developed by anther, which serves as materials for isolating and cloning Rf3 gene by using of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) we had improved in this study.

该特异蛋白质点的出现与消失可能与玉米CMS-S的育性恢复有一定关系，对其纯化分离将为Rf3基因的克隆奠定基础和进一步了解玉米质核互作不育表达模式的分子机制。

Near isogenic lines were the best populations for QTL fine mapping and positional cloning,A few studies had been reported their results on materials with improvement traits using

本文从QTL的定位群体，定位方法，研究现状，精细定位与克隆，以及QTL利用等方面对作物数量性状基因的研究进行了综述。

Representational difference analysis of cDNA is a powerful technique for cloning the differentially expressed genes between samples. Kandelia candel and Dunaliella salina are two important halophytes.

cDNA RDA技术是目前应用较为广泛的一种分离差异表达基因的方法，盐藻和秋茄是两种重要的盐生植物。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。