查询词典 cloning

The cloning of corresponding biosynthetic gene clusters is essential for new drug discovery and yield improvement by metabolic engineering.

微生物产生众多结构和生物活性多样的次级代谢产物，其生物合成基因簇的克隆是药物创新和产量提高的必要前提。

Cloning of corresponding biosynthetic gene clusters is essential for new drug discovery and yield improvement by metabolic engineering.

微生物产生众多结构和生物活性多样的次级代谢产物，其生物合成基因簇的克隆是药物创新和产量提高的必要前提。

All the results were essential for the cloning of cytosinpeptidemycin biosynthetic genes and illustrating the total biosynthesis.

以上工作对克隆获得完整的嘧肽霉素生物合成基因簇、研究嘧肽霉素的生物合成途径具有重要意义。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Boophilus microplus ; Bm86 gene ; cloning ; recombinant express vector

微小牛蜱； Bm86基因；克隆；重组表达载体

Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046, China）Abstract: With cDNA from salivary gland of semi-engorged adult female Boophilus microplus as tester and cDNA from starved adult female B.microplus as driver,a subtractive cDNA library of salivary gland of female B.microplus was constructed using suppression subtractive hybridization and T/A cloning.

以我国微小牛蜱半饱血雌性成蜱唾液腺cDNA为检测子Tester，饥饿雌性成蜱cDNA为驱动子，采用抑制消减杂交技术和T/A克隆技术构建了微小牛蜱半饱血雌性成蜱唾液腺消减cDNA文库。

In this paper, the cloning, expression and phylogenetic analysis of AmphiSDHD and AmphiS27 from the gut cDNA library of amphioxus Branchiostoma becheri tisingtauense are carried out.AmphiSDHD, encoding the cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase, was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense.

本文从青岛文昌鱼(Branchiostoma belcheri tsingtaunese)肠cDNA文库中，分离得到克隆L095和094，经鉴定它们分别编码文昌鱼线粒体琥珀酸-泛醌氧化还原酶中细胞色素b的小亚基和核糖体蛋白S27(AmphiS27)。

Kansas Senator Sam Brownback is a sponsor of a bill that outlaws the cloning of human embryos for research, because he says it's a life form that is eventually destroyed to harvest stem cells, and in his view that makes it abortion.

布朗柏科是这一个立案的提案人，立案为禁止因研究为目的的人类胚胎的复制，原因是他认为人类胚胎已经是生命了，而且那最终就是对于有收获的干细胞的摧毁，按照他的观点，那就是如同堕胎。

Very virulent infectious bursal disease virus; full-length cDNA ; genomic B segment ; cloning and sequence analysis

鸡传染性法氏囊病病毒超强毒；病毒基因组cDNA ；全长基因组B片段；序列分析

Parvifolia was 642bp by cloning PCR production and sequencing, and its phylogenetic information might facilitate to provide the direct molecular evidence to clarify its origion and evolution for Buxus sinica var. parvifolia.

珍珠黄杨ITS片段克隆测序后获得的序列长度为642bp，其系统学信息将为珍珠黄杨的起源进化提供有力的分子水平证据。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。