查询词典 cloning

To study the molecular structure and function of the PyNPase, and to construct a system for preparation of the enzyme for practical synthesis of nucleoside analogs, molecular cloning of the encoding the PyNPase from E. aerogenes strain EAM-Z〓 have now been completed.

我们通过以该酶的N端氨基酸序列设计上游引物，以不同来源PyNPase的C末端同源区氨基酸序列设计下游引物，采用PCR技术，扩增出该酶基因的编码区（约1206bp），并完成了其重组质粒的构建和核苷酸序列的测定，目前正在进行PyNPase表达质粒的构建。

Imidase ; Gene cloning ; Sequence analysis ; Gene expression ; Alcaligenes eutrophus

酰亚胺酶；基因克隆；序列分析；基因表达；真养产碱杆菌

Molecular cloning and characterization of the novel lection gene. Primers were designed based on conserved sequences of other lectin genes from Amaryllidaceae. A novel lectin gene was isolated from Zephyrathes grandiflora. The full-length cDNA of Zephyrathes grandiflora lectin was 955bp, containing an open reading frame (576bp) encoding a peptide of 191 amino acids. The gene was named as zga (Zephyrathes grandiflora agglutinin), with its encoding protein showing 59% similarity to GNA.

基因克隆及序列分析：根据石蒜科植物凝集素的保守区段，设计引物，采用RACE技术从韭莲中克隆出长度为952bp的新凝集素基因，包含了完整的编码序列(576bp)，它编码一个全长191个氨基酸的蛋白，与雪花莲外源凝集的相似性达59％，被命名为ZGA(Zephyrathes grandiflora agglutinin)。

The cloning strains would be died in HAT selective medium of triple aminopurine.

培养驯化的13株克隆虫株，经6-巯基鸟嘌呤(6-TG)抗性测定，10株存活。

We cloned the Rana temporaria chensinensis David skin cDNA using a PCR strategy and detected its activity in rana using a antibacterial protein assay against Micrococcus luteus. The cloning was based on a report of GenBank. The deduced amino acid sequence resulted in 53 amino with 46% identity to amphibia. RT-PCR was used to detect peptide mRNA in Rana temporaria chensinensis David.

我们用提取中国林蛙皮肽总RNA，逆转录合成cDNA，PCR扩增得到DNA片段，并与GenBank上登陆的其他蛙类的皮肽抗菌肽序列进行比较，发现此序列是一个新的蛙皮肽抗菌肽序列。

Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测，同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

This research has been based upon Aspergillus niger MC062, cloning and the expression of xylanase gene .

本研究对黑曲霉MC062的木聚糖酶基因进行了克隆与酿酒酵母表达。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

And genetic analysis and mapping of avirulence genes from the cross between M. grisea isolates were also studied for aiding to the positional cloning and further functional studies of avirulence genes.A total of 482 isolates were collected from 13 areas in China. Each isolate was subjected to DNA fingerprint analysis using pot2-rep-PCR.

同时，通过获得与稻瘟病菌无毒基因紧密连锁的分子标记，不仅可利用该无毒基因的标记作探针，研究该无毒基因在自然群体中的分布情况，揭示病菌群体毒性组成和变异特点，且为进一步物理作图法克隆该无毒基因奠定基础。

In 2001,the United State House of Representatives approved a bill banning all forms of human cloning.

在2001年，美国众议院批准了一项法令，是关于禁止所有形式的克隆人的法令。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。