查询词典 cloning

Molecular cloning and characterization of the novel lection gene. Primers were designed based on conserved sequences of other lectin genes from Amaryllidaceae. A novel lectin gene was isolated from Zephyrathes grandiflora. The full-length cDNA of Zephyrathes grandiflora lectin was 955bp, containing an open reading frame (576bp) encoding a peptide of 191 amino acids. The gene was named as zga (Zephyrathes grandiflora agglutinin), with its encoding protein showing 59% similarity to GNA.

基因克隆及序列分析：根据石蒜科植物凝集素的保守区段，设计引物，采用RACE技术从韭莲中克隆出长度为952bp的新凝集素基因，包含了完整的编码序列(576bp)，它编码一个全长191个氨基酸的蛋白，与雪花莲外源凝集的相似性达59％，被命名为ZGA(Zephyrathes grandiflora agglutinin)。

The cloning strains would be died in HAT selective medium of triple aminopurine.

培养驯化的13株克隆虫株，经6-巯基鸟嘌呤(6-TG)抗性测定，10株存活。

We cloned the Rana temporaria chensinensis David skin cDNA using a PCR strategy and detected its activity in rana using a antibacterial protein assay against Micrococcus luteus. The cloning was based on a report of GenBank. The deduced amino acid sequence resulted in 53 amino with 46% identity to amphibia. RT-PCR was used to detect peptide mRNA in Rana temporaria chensinensis David.

我们用提取中国林蛙皮肽总RNA，逆转录合成cDNA，PCR扩增得到DNA片段，并与GenBank上登陆的其他蛙类的皮肽抗菌肽序列进行比较，发现此序列是一个新的蛙皮肽抗菌肽序列。

Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测，同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

This research has been based upon Aspergillus niger MC062, cloning and the expression of xylanase gene .

本研究对黑曲霉MC062的木聚糖酶基因进行了克隆与酿酒酵母表达。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

And genetic analysis and mapping of avirulence genes from the cross between M. grisea isolates were also studied for aiding to the positional cloning and further functional studies of avirulence genes.A total of 482 isolates were collected from 13 areas in China. Each isolate was subjected to DNA fingerprint analysis using pot2-rep-PCR.

同时，通过获得与稻瘟病菌无毒基因紧密连锁的分子标记，不仅可利用该无毒基因的标记作探针，研究该无毒基因在自然群体中的分布情况，揭示病菌群体毒性组成和变异特点，且为进一步物理作图法克隆该无毒基因奠定基础。

In 2001,the United State House of Representatives approved a bill banning all forms of human cloning.

在2001年，美国众议院批准了一项法令，是关于禁止所有形式的克隆人的法令。

Although human cloning would further complicate the legal issues, this is not grounds for banning it.

虽然人的克隆将会进一步使法律问题复杂化，但这并不是禁止它的理由。

TPP gene plays an important role in synthesis of trehalose. The cloning of TPP gene lays a solid basis for investigating the biosynthesis of trehalose in L. bicolor.

海藻糖－6-磷酸磷酸酯酶在海藻糖合成过程中起关键作用，该基因的克隆为研究海藻糖在二色补血草中的合成奠定了基础。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。