查询词典 cloning

Through the comparisons, it was observed that there were extensive conserved relationships of maize QTL affected plant height, row number, and kernels per row with rice QTL affected plant height, tillers per plant, and grains per panicle respectively. 16 of 45 QTL affecting five different maize traits were conserved compared with 12 of 38 QTL affecting five different rice traits, which provided some useful information for locating, isolating and cloning maize QTL by using the rice genomic data.

研究结果表明：在玉米和水稻共线性的染色体区段，控制玉米株高、行数和行粒数的QTL与控制水稻株高、单株有效穗和每穗实粒数的QTL存在广泛的对应关系；在已定位的影响玉米株高等5个性状的45个QTL中，有16个与水稻"汕优63"群体中5个相同或相似性状所定位的38个QTL中的12个具有共线性关系。

In the last 20 years, cloning and expression of MLF related genes in Saccharomyces cerevisiae has been studied. Research progress and problems in this field are reviewed.

近20年来，围绕苹果酸-乳酸发酵相关基因的克隆以及在酿酒酵母中表达的研究取得了一些进展，本文就该研究进展和存在的问题进行了综述。

Sainfoin ; Tissue culture ; Somatic embryo ; Cloning

红豆草；组织培养；体细胞胚；克隆

PCRs were performed on genomic DNAs of four pear cultivars Sand pear, Huangguan pear, Xueli pear, Yali pear using primers derived from sequences of previous published PPO. The target bands were recovered and purified. The fragments were ligated with the plasmid of pGEM-T Vector by the method of direct T-A cloning.

以4种不同来源的梨基因组DNA为模板，根据已经发表的多酚氧化酶基因序列设计引物，利用PCR技术，克隆到了鸭梨、雪花梨、砂梨、黄冠梨约1.8 kb的完整多酚氧化酶基因，将纯化后的扩增产物克隆到质粒pGEM-T vec-tor中，转化DH-5α菌，挑选阳性克隆进行测序。

Keywords: gene cloning; cardiac-specific kinase; sarcomere; cardiac Troponin I; antioxidant protein-1

中文关键词：基因克隆；心肌特异激酶；肌小节；心肌肌钙蛋白I；抗过氧化物蛋白

The successful cloning of these isoenzyme genes of sea snake PLA2 may provide new information for the study on structure-function relationship of PLA2 family and its possible molecular mechanism.

这组海蛇PLA2同功酶基因的成功克隆为进一步揭示PLA2的结构与功能关系及可能的分子机制提供了新的信息。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体，在大肠杆菌 BL21（DE3）中进行诱导表达，并对表达产物进行纯化和复性，以获得大量ESAT-6P1抗原，同时对重组多免疫反应性进行鉴定评价，为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

The development of gene targeting and animal cloning thechnology makes the site-specific integration of foreign gene become possible.

随着基因打靶技术和动物克隆技术技术的发展，外源基因在高等哺乳动物基因组中的定点整合成为了可能。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。