查询词典 cloning

Sainfoin ; Tissue culture ; Somatic embryo ; Cloning

红豆草；组织培养；体细胞胚；克隆

PCRs were performed on genomic DNAs of four pear cultivars Sand pear, Huangguan pear, Xueli pear, Yali pear using primers derived from sequences of previous published PPO. The target bands were recovered and purified. The fragments were ligated with the plasmid of pGEM-T Vector by the method of direct T-A cloning.

以4种不同来源的梨基因组DNA为模板，根据已经发表的多酚氧化酶基因序列设计引物，利用PCR技术，克隆到了鸭梨、雪花梨、砂梨、黄冠梨约1.8 kb的完整多酚氧化酶基因，将纯化后的扩增产物克隆到质粒pGEM-T vec-tor中，转化DH-5α菌，挑选阳性克隆进行测序。

Keywords: gene cloning; cardiac-specific kinase; sarcomere; cardiac Troponin I; antioxidant protein-1

中文关键词：基因克隆；心肌特异激酶；肌小节；心肌肌钙蛋白I；抗过氧化物蛋白

The successful cloning of these isoenzyme genes of sea snake PLA2 may provide new information for the study on structure-function relationship of PLA2 family and its possible molecular mechanism.

这组海蛇PLA2同功酶基因的成功克隆为进一步揭示PLA2的结构与功能关系及可能的分子机制提供了新的信息。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体，在大肠杆菌 BL21（DE3）中进行诱导表达，并对表达产物进行纯化和复性，以获得大量ESAT-6P1抗原，同时对重组多免疫反应性进行鉴定评价，为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

The development of gene targeting and animal cloning thechnology makes the site-specific integration of foreign gene become possible.

随着基因打靶技术和动物克隆技术技术的发展，外源基因在高等哺乳动物基因组中的定点整合成为了可能。

First, Human-cloning will be confronted with great challenges to human signification, changing human biologically, psychologically and sociologically.

首先，克隆人将使人的涵义面临严峻挑战，改变人的生物学层面、心理学层面、社会学层面。

Methods Based on the cloning of human sodium/iodide, stably expressing hNIS human glioma cell lines TJ-905-t and transient co-transfection of hNIS and hT-PO cell lines TJ-905-co were obtained, their radioactive 125I uptake function and ^131I inhibitory effect on cell proliferation were investigated.

方法应用基因转染技术获得稳定表达hNIS的TJ-905转以及hNIS和人甲状腺过氧化物酶瞬时共转染的TJ-905共，然后研究两种细胞的摄碘功能以及^131I的细胞毒作用。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。