查询词典 cloning

Cloning and sequencing of the genes coding a and 3 subunit of phycoerythrin of a red alga-Corallina officinalis are reported firstly(GenBankaccession number: AF510986). The sequence contain 1,140 nucleotide, including 10 nucleotide that locate in the upstream of the initiation codon ofP subunit, 534 coding for P subunit(coding 177 amino acid), 101 nucleotide for gene spacer and 495 nucleotide that draw near the 5 terminal of a subunit(coding 164 amino acid).

首次测定了珊瑚藻藻红蛋白α和β亚基基因的全序列(GenBank登录号：AF510986)，测定的基因序列长为1，140个碱基，包括β亚基起始密码子上游的10个碱基，6亚基编码区的534个碱基(编码177个氨基酸)，101个碱基的间隔区以及α亚基编码区近5'端的495个碱基(编码164个氨基酸)。

It is of great importance to find out the antigenic epitope of TRP-2 as an antigen in pigmental cells, hi order to set up a foundation to investigate the epitope of TRP-2, we studied on the cloning and expression of human TRP -2 code gene.

确定其抗原表位对于深入研究该抗原及临床应用具有极其重要的意义，为了给TRP—2抗原表位确定的研究打下基础，本研究克隆了人TRP—2编码基因并表达了TRP—2蛋白。

Avian Escherichia coli ; type 1 pili ; structural gene ; cloning

鸡大肠杆菌； 1型菌毛；结构基因；克隆

These QTLs, particularly qNFGP-3-2 and qTNSP-3 with stronger additive effects and higher contribution rates would be further studied for fine-scale mapping and/or cloning. Some intervals with QTL cluster were determined in QTL mapping and most of the QTLs tended to have pleiotropism.

这些QTL，尤其是第3染色体RM168-RM143区间控制每穗总粒数的qTNSP-3和控制每穗实粒数的qNFGP-3-2，其加性效应值和贡献率均较大，可以考虑下一步进行QTL精细定位和克隆。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Cleavage polyembryony The splitting of an embryo into several identical parts, each of which may develop into a mature embryo. It is form of natural cloning.

裂生多胚现象：一个受精卵在发育过程中胚原细胞分裂为几个胚的现象，是自然克隆的一种形式。

Progress in cloning techniques, microbiology and protein purification has been supplying an ever-growing number and quantity of preparatively useful biocatalysts. Initially enzymes were only used to synthesize compounds found in nature.

近年来人们在克隆技术，微生物学以及蛋白纯化方面的研究成果为我们提供了越来越多的生物催化剂，酶最初用于天然产物的合成，而今其在非天然化合物中的应用更引起了生物化学家的广泛关注。

The essence of animal cloning is an adverse process of cell differentiation.

克隆动物的实质是细胞分化过程的逆转。

In the present research, the cloning of human FA-1 gene and study on 5' and 3' MAR of protamine genes had performed.

有鉴于此，本文对人FA-1基因及精蛋白基因5'端、3'端核基质结合区作了如下研究。

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆虫的两类AChE严格的区分，其中Ⅰ型AChE先与脊椎动物AChE聚合后才与Ⅱ型AChE相聚，说明两类AChE基因在物种分化前就已经分化。4嗜卷书虱烟碱型乙酰胆碱受体基因克隆及其mRNA表达水平研究利用RT-PCR和RACE技术成功克隆获得了嗜卷书虱2个烟碱型乙酰胆碱受体亚基的全长序列，分别命名为Lb a1(GenBank登录号：EU871527)和Lb a8(GenBank登录号：EU871526)。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。