查询词典 cloning

Human cytomegalovirus ; Phosphoprotein ; Molecular cloning ; Restriction site

人巨细胞病毒；磷蛋白；分子克隆；限制性酶切位点

Cloning and sequencing of the phycocyanin gene from Spirulina maxima and its evolutionary analysis.

极大螺旋藻藻蓝蛋白基因的克隆及其进化分析。

Cloning and sequencing of the genes coding a and 3 subunit of phycoerythrin of a red alga-Corallina officinalis are reported firstly(GenBankaccession number: AF510986). The sequence contain 1,140 nucleotide, including 10 nucleotide that locate in the upstream of the initiation codon ofP subunit, 534 coding for P subunit(coding 177 amino acid), 101 nucleotide for gene spacer and 495 nucleotide that draw near the 5 terminal of a subunit(coding 164 amino acid).

首次测定了珊瑚藻藻红蛋白α和β亚基基因的全序列(GenBank登录号：AF510986)，测定的基因序列长为1，140个碱基，包括β亚基起始密码子上游的10个碱基，6亚基编码区的534个碱基(编码177个氨基酸)，101个碱基的间隔区以及α亚基编码区近5'端的495个碱基(编码164个氨基酸)。

It is of great importance to find out the antigenic epitope of TRP-2 as an antigen in pigmental cells, hi order to set up a foundation to investigate the epitope of TRP-2, we studied on the cloning and expression of human TRP -2 code gene.

确定其抗原表位对于深入研究该抗原及临床应用具有极其重要的意义，为了给TRP—2抗原表位确定的研究打下基础，本研究克隆了人TRP—2编码基因并表达了TRP—2蛋白。

Avian Escherichia coli ; type 1 pili ; structural gene ; cloning

鸡大肠杆菌； 1型菌毛；结构基因；克隆

These QTLs, particularly qNFGP-3-2 and qTNSP-3 with stronger additive effects and higher contribution rates would be further studied for fine-scale mapping and/or cloning. Some intervals with QTL cluster were determined in QTL mapping and most of the QTLs tended to have pleiotropism.

这些QTL，尤其是第3染色体RM168-RM143区间控制每穗总粒数的qTNSP-3和控制每穗实粒数的qNFGP-3-2，其加性效应值和贡献率均较大，可以考虑下一步进行QTL精细定位和克隆。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Cleavage polyembryony The splitting of an embryo into several identical parts, each of which may develop into a mature embryo. It is form of natural cloning.

裂生多胚现象：一个受精卵在发育过程中胚原细胞分裂为几个胚的现象，是自然克隆的一种形式。

Progress in cloning techniques, microbiology and protein purification has been supplying an ever-growing number and quantity of preparatively useful biocatalysts. Initially enzymes were only used to synthesize compounds found in nature.

近年来人们在克隆技术，微生物学以及蛋白纯化方面的研究成果为我们提供了越来越多的生物催化剂，酶最初用于天然产物的合成，而今其在非天然化合物中的应用更引起了生物化学家的广泛关注。

The essence of animal cloning is an adverse process of cell differentiation.

克隆动物的实质是细胞分化过程的逆转。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。