查询词典 cloning

In this research we obtained the banana ACO gene with about 1.2kb length by cuffing from the cloning vector pUCm-ACO with restriction endonucleases Xba Ⅰ and Sac Ⅰ, and then directionally linked the gene to the plasmid vector pBⅠ-121 in the same endonucleases digestion sites, finally we got the construct of plant expression vector pBI-aACO.

用限制性内切酶Xba Ⅰ和sac Ⅰ从克隆载体pUCm-ACO上切下约1.2kb的香蕉ACO基因，将其定向连接在经相同酶切的质粒载体pBⅠ-121上，构建成植物表达载体pBⅠ-aACO。

Molecular Cloning and Sequence Analysis of two cDNA Fragments of Pheromone Binding Protein in Common Cutworm, Spodoptera litura Fabricius

斜纹夜蛾信息素结合蛋白cDNA片段的克隆及序列分析，修伟明；董双林-66文免费论文库，大学生论文库

The paper mainly reviewed several factors on influencing somatic cloning of pig , which involves in cytoplast recipients、 donor cells、micromanipulation、activation and reconstituted embryo transfer .

本文主要综述了影响体细胞克隆猪的几个因素，涉及胞质受体、供核细胞、显微操作、激活以及重组胚移植。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

The present study wastherefore designed to examine the molecular toxic effect of MC-LR on bighead carpAristichthys nobilis and zebrafish Danio rerio by using real-time polymerase chainreaction, molecular cloning and immunoblotting.

本研究以斑马鱼和鳙鱼为对象，采用实时荧光定量PCR、分子克隆和免疫印迹技术在分子水平上揭示了MC-LR对鱼类的毒理效应。

For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

For example, we cannot copy an unknown state exactly because of "the no-cloning theorem"(no-deleting theorem), which is known as one of the most significant differences between classical and quantum information. Therefore to get to know what we can and cannot do in QIS or in quantum physics becomes an important and interesting problem. Particularly, in quantum communication and quantum cryptography, many novel schemes are based on the fact that nonorthogonal states cannot be discriminated determinately.

例如，经典信息中，编码状态是可以精确复制、区分的；而在量子信息中，由于叠加性的存在，使得非正交态不可区分，量子态不可复制、删除等；故而以往既成的观念都被打破，探讨在量子信息的框架下编码信息的估计、物理可实现的操作，以及这些量子体系在外界通过物理方法进行识别过程中的差异，是量子信息学、乃至物理学中的重要也很基本的问题。

Now the research on HPL focus on dicotyledon, and the research on HPL of monocotyledon still havent been reported. In this paper, the research on cloning of rice HPL and enzyme activity assay were carried out and several result were obtained.1. Enzyme activity assay of rice HPLBased on the method of measure HPL Enzyme activity in other plants, the means on preparation of hydroperoxides and measurement rice HPL activity were set up. The 9-HPL arid 13-HPL activity of several rice varieties with different storability and different dormancy were tested.

本试验建立了水稻的HPL酶活性测定体系，测定了水稻萌发过程中的HPL酶活性，并进行了水稻HPL基因的克隆及表达研究，结果如下： 1、水稻HPL的酶活性测定在参考其它植物HPL酶活性测定方法的基础上，建立了水稻的HPL底物发生体系和酶活性测定体系，并测定了不同贮藏性水稻品种萌发过程中的种胚9-HPL、13-HPL活性和不同休眠性水稻品种种子的9-HPL、13-HPL酶活性。

Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in GNETIC ENGINEERING.

在许多双子叶植物中质粒在寄主细胞中是独立的复制单元，所以可以在基因工程中用作克隆载体。

There are many methods for the cloning of novel genes, but one of the most efficient methods is based on the diff...

新基因克隆的方法很多，利用细胞间基因表达的差异是克隆功能性新基因最有效的手段之一。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐