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Fragment as probe to hybridize with more than 30, 000 phage clones of a uamda gtlO library constructed from poiy~+RNA isolated from 48 hours NaCI (200mM) treated leaves from L. sinense. Several potential. phage clones were got and need further character i zat ion.

利用已构建的补血草的λ。gt10 cDNA文库（RNA来源：补血草幼苗用200mM NaCl处理48小时后提取叶片RNA），对三万多个噬菌体克隆分别用用136号片段做探针进行杂交筛选，得到了一些阳性克隆，进一步的鉴定工作仍在进行中。

A human MDRl full length cDNA probe was used to screen a pufferfish cosmid library and nine clones, which would contain the whole MDR gene family in the pufferfish genome, were finally identified. One of the cosmid clones was sequenced. Sequence analysis revealed that there would be at least three MDR homologues in the corresponding region.

其表达产物为一跨膜蛋白p-glycoprotein，通过ATP提供能量，将进入细胞内的多种物质泵出细胞外，从而使细胞对这些物质产生耐药性，多药耐药基因的功能决定了它在肿瘤化疗中的位置而使其成为基因结构、功能和表达、调控研究中的热点。

NaCl-tolerant clones of Z. sinica and Z. matrella (LRM and LCG, could retain a few green leaves at 7.5% NaCl for 3 weeks) and two sensitive clones of Z. matrella (HB and KE, showed 100% and near 100% leaf necroses in 3% NaCl) were used for this study. Plants were grown in nutrient solution supplemented with NaCl up to 3% under controlled conditions.

本研究选取耐盐性高(LRM及LCG，在7.5% NaCl浓下培养三周尚能维持一些)及低(HB及KE，在3% NaCl浓下培养三周后，片全枯及近於全枯)者各二营养系为材，将之培养於含有NaCl之营养液中，浓从0.5%渐增至3%，探讨其在盐逆境下之生反应。

The Kaminoans reconditioned an average of seven aberrant clones for every two hundred clones.

在卡米诺人的控制下，平均每两百个复制人会产生七个异常。

There are several clones or semi-clones of the TS series on the market.

有几个无性或半无性的TS系列的市场前景。

Out of 20 clones integrated with ibeB, two clones were investigated in detail.

对其中的2个进行插入基因表达的鉴定，为ibeB基因表达的阳性克隆。

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

Results Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.

所获得的51个克隆序列属于39个OTUs，其中52.9%的克隆序列分布于放线菌门放线菌亚的5个亚目和酸微菌亚纲中，并且在这两个亚纲中有大量克隆序列属于放线菌的新类群，另外47.1%的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支，极有可能代表一个新亚目或更高级分类单元的类群。

The twig production of different clones is different, from 5.5 twigs per month to 14.8 twigs per month. The rooting rate, root number and root length change regularly as the concentration of IBA goes up from 0.05 to 2.00 mg/L. At the concentration of 0.05 mg/L ppm IBA, rooting rate and root length are the highest. Cutting substrate also has eminent effect on rooting rate and survival rate, 50% peat plus 50% sub-top soil is the best substrate. The survival rate differs greatly among clones, from 56.7% to 90.0%. Rooting rate and survival rate change regularly as season changes. The rooting rate of May and June is middle, that of July and August is high and that of September and October drops rapidly. The survival rate is low in May, September and October and high in June, July and August.

结果表明：不同无性系的萌条数量有显著差异，高的达14.8条／月，低的5.5条／月，平均9.1条／月，从0.05~2.00 mg/L，随着IBA浓度的提离，生根率、根条数、总根长等生根指标均呈现有规律的变化；综合考虑各种生根指标，以IBA0.05 mg/L为最佳生根激素处理，扦插介质对柚木插穗生根有显著的影响，以泥炭土＋黄心土按1：1混合为最佳扦插介质；不同无性系的扦插成活率不同，从56.7%至90.0%，变动幅度大，随扦插季节变化，生根率和成活率均呈现有规律的变化，5~6月份的生根率中等，7~8月最高，9~10月份迅速下降，成活率随季节的变化也是两头低、中间高，6~8月的成活率最高，大于93%。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。