查询词典 clones

Dendritic cells are the most potent antigen-presenting cells in human immune system. Our institute has been working on the region of DC development and DC-mediated immune therapy on cancers for a long time. A cDNA library of human dendritic cells has been set up and by large-scared random sequencing, we have isolated a lot of novel fulllength cDNA clones.

树突状细胞(Dendritic cells，DC)是人体内重要的抗原提呈细胞，本研究所对DC的分化发育及其介导的肿瘤免疫治疗的研究工作已进行多年，并在此基础上建立了人树突状细胞基因文库，通过大规模随机测序的方法开展了从DC中克隆新分子的工作，从2万余条EST中发现了许多新的分子。

Were stored individually in 349 pieces of 384 microtiter plates, and they were gridded onto 116 filters (3×3×384 clones on 8×l2cm〓 filter) that can be used to screen by hybridization.

该文库是国内构建的第一个玉米BAC文库，所用材料是国内特有的玉米资源，为我国玉米基因组研究提供了技术平台。

While Baker already has a library of thousands of voices in his head, he says it's not difficult adding to the catalog and quickly referencing those intonations -- even when there are so many clones, each of whom is subtly different.

由于贝克的大脑如同一座有着数千种声音的图书馆，因此他说再多几种声音并且迅速引用那些语调并不是什么难事——即使有那么多的克隆人，每一个都有微妙的不同。

Dr. Maria Andrade joined CIP and continued to be based in Maputo focusing on sweetpotato breeding, seed systems, and value chain development. In 2007, Dr. Paul Demo from his base in Malawi began to directly collaborate with IIAM's potato program in adaptive testing of late-blight resistant potato clones and the improvement of seed supply. In 2006, an agricultural economist, Dr.

玛丽亚博士安德拉德加入国际马铃薯中心和商业化将继续设在马普托重点甘薯育种，种子系统和价值链的发展。2007年，保罗医生从他的示范基地，开始在马拉维直接协作 IIAM 在晚马铃薯抗晚疫病无性系适应性测试计划和马铃薯种子的改善供应。2006年，农业经济学家，博士里卡多拉瓦尔塔，被派往赞比西亚省，协调种子系统的达成最终用户组成的项目如下。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

Only a small percentage of clones result in pregnancies and there is a high percentage of miscarriages and deformities.

能够让实验对象怀孕的几率非常小，而且怀孕后流产和畸形胎儿的几率也相当高。

Human clones, it is widely assumed, would be monstrous perversions of

人们普遍以为，人类克隆将是对大自然的怪异歪曲。

Human clones , it is widely assumed, would be monstrous perversions of nature.

人们普遍以为，人类克隆将是对大自然的怪异歪曲。

Results and Conclusion Five positive clones were obtained by immunological screening of 5 x 10^4 recombinants.

经PCR扩增并测序分析表明，5个阳性克隆基因与GenBank中已知基因无明显同源性。

The two clones derived from TP7+AG1 which carried both Sal Ⅰ and Not Ⅰ markers were found that they are likely to be the recombinants.

来源于TP7+AGl的2个带有SalⅠ和NotⅠ双酶切标记的两个克隆通过序列测定发现可能是CCMV-RNA3的重组子。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。